Pbp1p, a factor interacting with Saccharomyces cerevisiae poly(A)-binding protein, regulates polyadenylation
UMass Chan Affiliations
Department of Molecular Genetics and MicrobiologyDocument Type
Journal ArticlePublication Date
1998-11-20Keywords
Amino Acid SequenceCarrier Proteins
Cloning, Molecular
Fungal Proteins
Gene Deletion
Gene Expression Regulation, Fungal
Genes, Fungal
Genes, Reporter
Molecular Sequence Data
Nuclear ProteinsOligonucleotidesPoly APoly(A)-Binding ProteinsPolyribosomesProtein BiosynthesisRNA, MessengerRNA-Binding ProteinsSaccharomyces cerevisiae*Saccharomyces cerevisiae ProteinsSequence AlignmentSequence Analysis, DNALife SciencesMedicine and Health Sciences
Metadata
Show full item recordAbstract
The poly(A) tail of an mRNA is believed to influence the initiation of translation, and the rate at which the poly(A) tail is removed is thought to determine how fast an mRNA is degraded. One key factor associated with this 3'-end structure is the poly(A)-binding protein (Pab1p) encoded by the PAB1 gene in Saccharomyces cerevisiae. In an effort to learn more about the functional role of this protein, we used a two-hybrid screen to determine the factor(s) with which it interacts. We identified five genes encoding factors that specifically interact with the carboxy terminus of Pab1p. Of a total of 44 specific clones identified, PBP1 (for Pab1p-binding protein) was isolated 38 times. Of the putative interacting genes examined, PBP1 promoted the highest level of resistance to 3-aminotriazole (>100 mM) in constructs in which HIS3 was used as a reporter. We determined that a fraction of Pbp1p cosediments with polysomes in sucrose gradients and that its distribution is very similar to that of Pab1p. Disruption of PBP1 showed that it is not essential for viability but can suppress the lethality associated with a PAB1 deletion. The suppression of pab1Delta by pbp1Delta appears to be different from that mediated by other pab1 suppressors, since disruption of PBP1 does not alter translation rates, affect accumulation of ribosomal subunits, change mRNA poly(A) tail lengths, or result in a defect in mRNA decay. Rather, Pbp1p appears to function in the nucleus to promote proper polyadenylation. In the absence of Pbp1p, 3' termini of pre-mRNAs are properly cleaved but lack full-length poly(A) tails. These effects suggest that Pbp1p may act to repress the ability of Pab1p to negatively regulate polyadenylation.Source
Mol Cell Biol. 1998 Dec;18(12):7383-96.Permanent Link to this Item
http://hdl.handle.net/20.500.14038/38580PubMed ID
9819425Related Resources
Link to Article in PubMedCollections
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