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dc.contributor.authorBurns, Loree Griffin
dc.contributor.authorPeterson, Craig L.
dc.date2022-08-11T08:09:33.000
dc.date.accessioned2022-08-23T16:35:32Z
dc.date.available2022-08-23T16:35:32Z
dc.date.issued1997-08-01
dc.date.submitted2009-03-24
dc.identifier.citationMol Cell Biol. 1997 Aug;17(8):4811-9.
dc.identifier.issn0270-7306 (Print)
dc.identifier.pmid9234737
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38583
dc.description.abstractThe Saccharomyces cerevisiae SWI-SNF complex is a 2-MDa protein assembly that is required for the function of many transcriptional activators. Here we describe experiments on the role of the SWI-SNF complex in activation of transcription by the yeast activator GAL4. We find that while SWI-SNF activity is not required for the GAL4 activator to bind to and activate transcription from nucleosome-free binding sites, the complex is required for GAL4 to bind to and function at low-affinity, nucleosomal binding sites in vivo. This SWI-SNF dependence can be overcome by (i) replacing the low-affinity sites with higher-affinity, consensus GAL4 binding sequences or (ii) placing the low-affinity sites into a nucleosome-free region. These results define the criteria for the SWI-SNF dependence of gene expression and provide the first in vivo evidence that the SWI-SNF complex can regulate gene expression by modulating the DNA binding of an upstream activator protein.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=9234737&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectBinding Sites
dc.subjectChromosomal Proteins, Non-Histone
dc.subjectDNA Footprinting
dc.subjectDNA, Fungal
dc.subjectDNA-Binding Proteins
dc.subjectFungal Proteins
dc.subjectGalactose
dc.subjectGene Expression Regulation, Fungal
dc.subjectGenes, Fungal
dc.subject*Nuclear Proteins
dc.subjectNucleosomes
dc.subjectPromoter Regions (Genetics)
dc.subjectSaccharomyces cerevisiae
dc.subject*Saccharomyces cerevisiae Proteins
dc.subjectTrans-Activation (Genetics)
dc.subjectTrans-Activators
dc.subjectTranscription Factors
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleThe yeast SWI-SNF complex facilitates binding of a transcriptional activator to nucleosomal sites in vivo
dc.typeJournal Article
dc.source.journaltitleMolecular and cellular biology
dc.source.volume17
dc.source.issue8
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2448&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1449
dc.identifier.contextkey794946
refterms.dateFOA2022-08-23T16:35:33Z
html.description.abstract<p>The Saccharomyces cerevisiae SWI-SNF complex is a 2-MDa protein assembly that is required for the function of many transcriptional activators. Here we describe experiments on the role of the SWI-SNF complex in activation of transcription by the yeast activator GAL4. We find that while SWI-SNF activity is not required for the GAL4 activator to bind to and activate transcription from nucleosome-free binding sites, the complex is required for GAL4 to bind to and function at low-affinity, nucleosomal binding sites in vivo. This SWI-SNF dependence can be overcome by (i) replacing the low-affinity sites with higher-affinity, consensus GAL4 binding sequences or (ii) placing the low-affinity sites into a nucleosome-free region. These results define the criteria for the SWI-SNF dependence of gene expression and provide the first in vivo evidence that the SWI-SNF complex can regulate gene expression by modulating the DNA binding of an upstream activator protein.</p>
dc.identifier.submissionpathoapubs/1449
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentDepartment of Biochemistry and Molecular Biology
dc.source.pages4811-9


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