A mutation in the tRNA nucleotidyltransferase gene promotes stabilization of mRNAs in Saccharomyces cerevisiae
dc.contributor.author | Peltz, Stuart W. | |
dc.contributor.author | Donahue, Janet L. | |
dc.contributor.author | Jacobson, Allan | |
dc.date | 2022-08-11T08:09:33.000 | |
dc.date.accessioned | 2022-08-23T16:35:36Z | |
dc.date.available | 2022-08-23T16:35:36Z | |
dc.date.issued | 1992-12-01 | |
dc.date.submitted | 2009-03-24 | |
dc.identifier.citation | Mol Cell Biol. 1992 Dec;12(12):5778-84. | |
dc.identifier.issn | 0270-7306 (Print) | |
dc.identifier.pmid | 1448105 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/38598 | |
dc.description.abstract | To identify trans-acting factors involved in mRNA decay in the yeast Saccharomyces cerevisiae, we have begun to characterize conditional lethal mutants that affect mRNA steady-state levels. A screen of a collection of temperature-sensitive mutants identified ts352, a mutant that accumulated moderately stable and unstable mRNAs after a shift from 23 to 37 degrees C (M. Aebi, G. Kirchner, J.-Y. Chen, U. Vijayraghavan, A. Jacobson, N.C. Martin, and J. Abelson, J. Biol. Chem. 265:16216-16220, 1990). ts352 has a defect in the CCA1 gene, which codes for tRNA nucleotidyltransferase, the enzyme that adds 3' CCA termini to tRNAs (Aebi et al., J. Biol. Chem., 1990). In a shift to the nonpermissive temperature, ts352 (cca1-1) cells rapidly cease protein synthesis, reduce the rates of degradation of the CDC4, TCM1, and PAB1 mRNAs three- to fivefold, and increase the relative number of ribosomes associated with mRNAs and the overall size of polysomes. These results were analogous to those observed for cycloheximide-treated cells and are generally consistent with models that invoke a role for translational elongation in the process of mRNA turnover. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=1448105&dopt=Abstract">Link to Article in PubMed</a> | |
dc.subject | Cycloheximide | |
dc.subject | Fungal Proteins | |
dc.subject | Kinetics | |
dc.subject | Mutation | |
dc.subject | Polyribosomes | |
dc.subject | Protein Biosynthesis | |
dc.subject | RNA Nucleotidyltransferases | |
dc.subject | RNA, Fungal | |
dc.subject | RNA, Messenger | |
dc.subject | RNA, Transfer | |
dc.subject | Saccharomyces cerevisiae | |
dc.subject | Temperature | |
dc.subject | Life Sciences | |
dc.subject | Medicine and Health Sciences | |
dc.title | A mutation in the tRNA nucleotidyltransferase gene promotes stabilization of mRNAs in Saccharomyces cerevisiae | |
dc.type | Journal Article | |
dc.source.journaltitle | Molecular and cellular biology | |
dc.source.volume | 12 | |
dc.source.issue | 12 | |
dc.identifier.legacyfulltext | https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2461&context=oapubs&unstamped=1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/oapubs/1462 | |
dc.identifier.contextkey | 794959 | |
refterms.dateFOA | 2022-08-23T16:35:36Z | |
html.description.abstract | <p>To identify trans-acting factors involved in mRNA decay in the yeast Saccharomyces cerevisiae, we have begun to characterize conditional lethal mutants that affect mRNA steady-state levels. A screen of a collection of temperature-sensitive mutants identified ts352, a mutant that accumulated moderately stable and unstable mRNAs after a shift from 23 to 37 degrees C (M. Aebi, G. Kirchner, J.-Y. Chen, U. Vijayraghavan, A. Jacobson, N.C. Martin, and J. Abelson, J. Biol. Chem. 265:16216-16220, 1990). ts352 has a defect in the CCA1 gene, which codes for tRNA nucleotidyltransferase, the enzyme that adds 3' CCA termini to tRNAs (Aebi et al., J. Biol. Chem., 1990). In a shift to the nonpermissive temperature, ts352 (cca1-1) cells rapidly cease protein synthesis, reduce the rates of degradation of the CDC4, TCM1, and PAB1 mRNAs three- to fivefold, and increase the relative number of ribosomes associated with mRNAs and the overall size of polysomes. These results were analogous to those observed for cycloheximide-treated cells and are generally consistent with models that invoke a role for translational elongation in the process of mRNA turnover.</p> | |
dc.identifier.submissionpath | oapubs/1462 | |
dc.contributor.department | Department of Molecular Genetics and Microbiology | |
dc.source.pages | 5778-84 |