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dc.contributor.authorPeltz, Stuart W.
dc.contributor.authorDonahue, Janet L.
dc.contributor.authorJacobson, Allan
dc.date2022-08-11T08:09:33.000
dc.date.accessioned2022-08-23T16:35:36Z
dc.date.available2022-08-23T16:35:36Z
dc.date.issued1992-12-01
dc.date.submitted2009-03-24
dc.identifier.citationMol Cell Biol. 1992 Dec;12(12):5778-84.
dc.identifier.issn0270-7306 (Print)
dc.identifier.pmid1448105
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38598
dc.description.abstractTo identify trans-acting factors involved in mRNA decay in the yeast Saccharomyces cerevisiae, we have begun to characterize conditional lethal mutants that affect mRNA steady-state levels. A screen of a collection of temperature-sensitive mutants identified ts352, a mutant that accumulated moderately stable and unstable mRNAs after a shift from 23 to 37 degrees C (M. Aebi, G. Kirchner, J.-Y. Chen, U. Vijayraghavan, A. Jacobson, N.C. Martin, and J. Abelson, J. Biol. Chem. 265:16216-16220, 1990). ts352 has a defect in the CCA1 gene, which codes for tRNA nucleotidyltransferase, the enzyme that adds 3' CCA termini to tRNAs (Aebi et al., J. Biol. Chem., 1990). In a shift to the nonpermissive temperature, ts352 (cca1-1) cells rapidly cease protein synthesis, reduce the rates of degradation of the CDC4, TCM1, and PAB1 mRNAs three- to fivefold, and increase the relative number of ribosomes associated with mRNAs and the overall size of polysomes. These results were analogous to those observed for cycloheximide-treated cells and are generally consistent with models that invoke a role for translational elongation in the process of mRNA turnover.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=1448105&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectCycloheximide
dc.subjectFungal Proteins
dc.subjectKinetics
dc.subjectMutation
dc.subjectPolyribosomes
dc.subjectProtein Biosynthesis
dc.subjectRNA Nucleotidyltransferases
dc.subjectRNA, Fungal
dc.subjectRNA, Messenger
dc.subjectRNA, Transfer
dc.subjectSaccharomyces cerevisiae
dc.subjectTemperature
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleA mutation in the tRNA nucleotidyltransferase gene promotes stabilization of mRNAs in Saccharomyces cerevisiae
dc.typeJournal Article
dc.source.journaltitleMolecular and cellular biology
dc.source.volume12
dc.source.issue12
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2461&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1462
dc.identifier.contextkey794959
refterms.dateFOA2022-08-23T16:35:36Z
html.description.abstract<p>To identify trans-acting factors involved in mRNA decay in the yeast Saccharomyces cerevisiae, we have begun to characterize conditional lethal mutants that affect mRNA steady-state levels. A screen of a collection of temperature-sensitive mutants identified ts352, a mutant that accumulated moderately stable and unstable mRNAs after a shift from 23 to 37 degrees C (M. Aebi, G. Kirchner, J.-Y. Chen, U. Vijayraghavan, A. Jacobson, N.C. Martin, and J. Abelson, J. Biol. Chem. 265:16216-16220, 1990). ts352 has a defect in the CCA1 gene, which codes for tRNA nucleotidyltransferase, the enzyme that adds 3' CCA termini to tRNAs (Aebi et al., J. Biol. Chem., 1990). In a shift to the nonpermissive temperature, ts352 (cca1-1) cells rapidly cease protein synthesis, reduce the rates of degradation of the CDC4, TCM1, and PAB1 mRNAs three- to fivefold, and increase the relative number of ribosomes associated with mRNAs and the overall size of polysomes. These results were analogous to those observed for cycloheximide-treated cells and are generally consistent with models that invoke a role for translational elongation in the process of mRNA turnover.</p>
dc.identifier.submissionpathoapubs/1462
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.source.pages5778-84


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