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dc.contributor.authorKirber, Michael T.
dc.contributor.authorEtter, Elaine F.
dc.contributor.authorBellve, Karl D.
dc.contributor.authorLifshitz, Lawrence M.
dc.contributor.authorTuft, Richard A.
dc.contributor.authorFay, Fredric S.
dc.contributor.authorWalsh, John V.
dc.contributor.authorFogarty, Kevin E.
dc.date2022-08-11T08:09:34.000
dc.date.accessioned2022-08-23T16:35:44Z
dc.date.available2022-08-23T16:35:44Z
dc.date.issued2001-03-07
dc.date.submitted2009-03-26
dc.identifier.citation<p>J Physiol. 2001 Mar 1;531(Pt 2):315-27.</p>
dc.identifier.issn0022-3751 (Print)
dc.identifier.pmid11230506
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38627
dc.description.abstractWe recorded Ca2+ sparks and spontaneous transient outward currents (STOCs) simultaneously in smooth muscle cells using whole-cell patch recording and a unique, high-speed widefield digital imaging system to monitor fluo-3 fluorescence in both two and three dimensions (2D and 3D). In 2D imaging, the correlation between the amplitude of a spark and its corresponding STOC was a weak one, and 27 % of the sparks failed to cause STOCs. The STOCless sparks were not significantly different in amplitude from those that caused STOCs. Three-dimensional imaging disclosed that STOCless sparks were located close to the cell surface, and on average their apparent distance from the cell surface was not significantly different from the sparks that cause STOCs. Statistical evaluation of spark clusters disclosed that there were regions of the cell where the probability of spark occurrence was high and others where it was quite low.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=11230506&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2278474/
dc.subjectAniline Compounds
dc.subjectAnimals
dc.subjectCalcium
dc.subjectCats
dc.subjectCell Membrane
dc.subjectElectric Conductivity
dc.subjectEsophagus
dc.subjectFluorescent Dyes
dc.subjectImage Processing, Computer-Assisted
dc.subjectImaging, Three-Dimensional
dc.subjectMuscle, Smooth
dc.subjectPatch-Clamp Techniques
dc.subjectXanthenes
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleRelationship of Ca2+ sparks to STOCs studied with 2D and 3D imaging in feline oesophageal smooth muscle cells
dc.typeJournal Article
dc.source.journaltitleThe Journal of physiology
dc.source.volume531
dc.source.issuePt 2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1489
dc.identifier.contextkey798464
html.description.abstract<p>We recorded Ca2+ sparks and spontaneous transient outward currents (STOCs) simultaneously in smooth muscle cells using whole-cell patch recording and a unique, high-speed widefield digital imaging system to monitor fluo-3 fluorescence in both two and three dimensions (2D and 3D). In 2D imaging, the correlation between the amplitude of a spark and its corresponding STOC was a weak one, and 27 % of the sparks failed to cause STOCs. The STOCless sparks were not significantly different in amplitude from those that caused STOCs. Three-dimensional imaging disclosed that STOCless sparks were located close to the cell surface, and on average their apparent distance from the cell surface was not significantly different from the sparks that cause STOCs. Statistical evaluation of spark clusters disclosed that there were regions of the cell where the probability of spark occurrence was high and others where it was quite low.</p>
dc.identifier.submissionpathoapubs/1489
dc.contributor.departmentDepartment of Physiology and Biomedical Imaging Group
dc.source.pages315-27


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