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    Mutational analysis of heptad repeats in the membrane-proximal region of Newcastle disease virus HN protein

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    Authors
    Stone-Hulslander, Judith
    Morrison, Trudy G.
    UMass Chan Affiliations
    Department of Molecular Genetics and Microbiology
    Document Type
    Journal Article
    Publication Date
    1999-04-10
    Keywords
    Alanine
    Amino Acid Sequence
    Amino Acid Substitution
    Animals
    Binding Sites
    COS Cells
    Gene Expression
    HN Protein
    Methionine
    *Microsatellite Repeats
    Molecular Sequence Data
    Mutagenesis, Site-Directed
    Newcastle disease virus
    Life Sciences
    Medicine and Health Sciences
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    Abstract
    For most paramyxoviruses, syncytium formation requires the expression of both surface glycoproteins (HN and F) in the same cell, and evidence suggests that fusion involves a specific interaction between the HN and F proteins (X. Hu et al., J. Virol. 66:1528-1534, 1992). The stalk region of the Newcastle disease virus (NDV) HN protein has been implicated in both fusion promotion and virus specificity of that activity. The NDV F protein contains two heptad repeat motifs which have been shown by site-directed mutagenesis to be critical for fusion (R. Buckland et al., J. Gen. Virol. 73:1703-1707, 1992; T. Sergel-Germano et al., J. Virol. 68:7654-7658, 1994; J. Reitter et al., J. Virol. 69:5995-6004, 1995). Heptad repeat motifs mediate protein-protein interactions by enabling the formation of coiled coils. Upon analysis of the stalk region of the NDV HN protein, we identified two heptad repeats. Secondary structure analysis of these repeats suggested the potential for these regions to form alpha helices. To investigate the importance of this sequence motif for fusion promotion, we mutated the hydrophobic a-position amino acids of each heptad repeat to alanine or methionine. In addition, hydrophobic amino acids in other positions were also changed to alanine. Every mutant protein retained levels of attachment activity that was greater than or equal to the wild-type protein activity and bound to conformation-specific monoclonal as well as polyclonal antisera. Neuraminidase activity was variably affected. Every mutation, however, showed a dramatic decrease in fusion promotion activity. The phenotypes of these mutant proteins indicate that individual amino acids within the heptad repeat region of the stalk domain of the HN protein are important for the fusion promotion activity of the protein. These data are consistent with the idea that the HN protein associates with the F protein via specific interactions between the heptad repeat regions of both proteins.
    Source
    J Virol. 1999 May;73(5):3630-7.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/38682
    PubMed ID
    10196255
    Related Resources
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    UMass Chan Faculty and Researcher Publications

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