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dc.contributor.authorMargolis, David M.
dc.contributor.authorSomasundaran, Mohan
dc.contributor.authorGreen, Michael R.
dc.date2022-08-11T08:09:34.000
dc.date.accessioned2022-08-23T16:36:03Z
dc.date.available2022-08-23T16:36:03Z
dc.date.issued1994-02-01
dc.date.submitted2009-03-26
dc.identifier.citationJ Virol. 1994 Feb;68(2):905-10.
dc.identifier.issn0022-538X (Print)
dc.identifier.pmid8289393
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38701
dc.description.abstractThe transcriptional activity of human immunodeficiency virus type 1 (HIV-1) is affected by many cellular factors. Homologies near the HIV-1 initiator region to the DNA-binding sequences of YY1, a multifunctional transcription factor known to regulate diverse viral and cellular promoters, suggested that YY1 might regulate HIV-1. Antibody to YY1 blocked the formation of complexes by HeLa cell nuclear extract and a DNA oligonucleotide encoding the HIV-1 initiator region. HIV-1 long terminal repeat (LTR) expression, as measured the expression of a transfected LTR-CAT reporter gene, was repressed more than 12-fold by the cotransfection of a YY1 expression vector. HIV-1 production by both COS-1 and CEM cells after transfection of an infectious molecular HIV-1 clone was repressed 7- to 20-fold by cotransfection of a YY1 expression vector. HIV-1 production was also decreased threefold in a CD4-positive lymphocyte cell line chronically infected with HIV-1 (8E5) after transfection of YY1. In situ hybridization studies confirmed that YY1 reduced HIV-1 RNA expression. YY1 may play an important role in the regulation of HIV-1 LTR expression in vivo and virus production by infected cells.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=8289393&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAnimals
dc.subjectBase Sequence
dc.subjectCell Line
dc.subjectChloramphenicol O-Acetyltransferase
dc.subjectDNA-Binding Proteins
dc.subjectErythroid-Specific DNA-Binding Factors
dc.subjectGene Expression Regulation, Viral
dc.subjectHIV Core Protein p24
dc.subjectHIV Long Terminal Repeat
dc.subjectHIV-1
dc.subjectHumans
dc.subjectIn Situ Hybridization
dc.subjectLymphoid Tissue
dc.subjectMolecular Sequence Data
dc.subjectProtein Binding
dc.subjectRNA, Messenger
dc.subjectRNA, Viral
dc.subjectRecombinant Fusion Proteins
dc.subjectTranscription Factors
dc.subjectTranscription, Genetic
dc.subjectVirion
dc.subjectYY1 Transcription Factor
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleHuman transcription factor YY1 represses human immunodeficiency virus type 1 transcription and virion production
dc.typeJournal Article
dc.source.journaltitleJournal of virology
dc.source.volume68
dc.source.issue2
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2554&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1555
dc.identifier.contextkey798532
refterms.dateFOA2022-08-23T16:36:03Z
html.description.abstract<p>The transcriptional activity of human immunodeficiency virus type 1 (HIV-1) is affected by many cellular factors. Homologies near the HIV-1 initiator region to the DNA-binding sequences of YY1, a multifunctional transcription factor known to regulate diverse viral and cellular promoters, suggested that YY1 might regulate HIV-1. Antibody to YY1 blocked the formation of complexes by HeLa cell nuclear extract and a DNA oligonucleotide encoding the HIV-1 initiator region. HIV-1 long terminal repeat (LTR) expression, as measured the expression of a transfected LTR-CAT reporter gene, was repressed more than 12-fold by the cotransfection of a YY1 expression vector. HIV-1 production by both COS-1 and CEM cells after transfection of an infectious molecular HIV-1 clone was repressed 7- to 20-fold by cotransfection of a YY1 expression vector. HIV-1 production was also decreased threefold in a CD4-positive lymphocyte cell line chronically infected with HIV-1 (8E5) after transfection of YY1. In situ hybridization studies confirmed that YY1 reduced HIV-1 RNA expression. YY1 may play an important role in the regulation of HIV-1 LTR expression in vivo and virus production by infected cells.</p>
dc.identifier.submissionpathoapubs/1555
dc.contributor.departmentDepartment of Pediatrics
dc.contributor.departmentProgram in Molecular Medicine
dc.source.pages905-10


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