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dc.contributor.authorLittaua, Rebecca A.
dc.contributor.authorOldstone, Michael B. A.
dc.contributor.authorTakeda, Akira
dc.contributor.authorEnnis, Francis A.
dc.date2022-08-11T08:09:34.000
dc.date.accessioned2022-08-23T16:36:07Z
dc.date.available2022-08-23T16:36:07Z
dc.date.issued1992-01-01
dc.date.submitted2009-03-26
dc.identifier.citationJ Virol. 1992 Jan;66(1):608-11.
dc.identifier.issn0022-538X (Print)
dc.identifier.pmid1370094
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38715
dc.description.abstractA CD4+ cytotoxic T-lymphocyte (CTL) clone, established from the peripheral blood of a human immunodeficiency virus (HIV)-seropositive donor, lysed autologous target cells that were infected with a recombinant vaccinia virus containing the gag gene of HIV type 1 and target cells pulsed with p24gag construct expressed in Escherichia coli. The recognition of the HLA-DQ-restricted epitope by this clone was further defined by using overlapping synthetic peptides. The epitope recognized by this CD4+ CTL clone (amino acids 140 to 148) overlaps with a CD8+ epitope and is highly conserved among all isolates of HIV type 1 that have been sequenced. Production and secretion of lymphokines such as interleukin-2 and interleukin-6 after specific antigenic stimulation were demonstrated by this gag-specific CD4+ CTL clone.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=1370094&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAmino Acid Sequence
dc.subjectCD4-Positive T-Lymphocytes
dc.subjectClone Cells
dc.subjectEpitopes
dc.subjectHIV Core Protein p24
dc.subjectHumans
dc.subjectInterleukin-2
dc.subjectInterleukin-6
dc.subjectMolecular Sequence Data
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleA CD4+ cytotoxic T-lymphocyte clone to a conserved epitope on human immunodeficiency virus type 1 p24: cytotoxic activity and secretion of interleukin-2 and interleukin-6
dc.typeJournal Article
dc.source.journaltitleJournal of virology
dc.source.volume66
dc.source.issue1
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2567&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1568
dc.identifier.contextkey798546
refterms.dateFOA2022-08-23T16:36:07Z
html.description.abstract<p>A CD4+ cytotoxic T-lymphocyte (CTL) clone, established from the peripheral blood of a human immunodeficiency virus (HIV)-seropositive donor, lysed autologous target cells that were infected with a recombinant vaccinia virus containing the gag gene of HIV type 1 and target cells pulsed with p24gag construct expressed in Escherichia coli. The recognition of the HLA-DQ-restricted epitope by this clone was further defined by using overlapping synthetic peptides. The epitope recognized by this CD4+ CTL clone (amino acids 140 to 148) overlaps with a CD8+ epitope and is highly conserved among all isolates of HIV type 1 that have been sequenced. Production and secretion of lymphokines such as interleukin-2 and interleukin-6 after specific antigenic stimulation were demonstrated by this gag-specific CD4+ CTL clone.</p>
dc.identifier.submissionpathoapubs/1568
dc.contributor.departmentCenter for Infectious Disease and Vaccine Research
dc.contributor.departmentDepartment of Medicine, Division of Infectious Diseases and Immunology
dc.source.pages608-11


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