A CD4+ cytotoxic T-lymphocyte clone to a conserved epitope on human immunodeficiency virus type 1 p24: cytotoxic activity and secretion of interleukin-2 and interleukin-6
dc.contributor.author | Littaua, Rebecca A. | |
dc.contributor.author | Oldstone, Michael B. A. | |
dc.contributor.author | Takeda, Akira | |
dc.contributor.author | Ennis, Francis A. | |
dc.date | 2022-08-11T08:09:34.000 | |
dc.date.accessioned | 2022-08-23T16:36:07Z | |
dc.date.available | 2022-08-23T16:36:07Z | |
dc.date.issued | 1992-01-01 | |
dc.date.submitted | 2009-03-26 | |
dc.identifier.citation | J Virol. 1992 Jan;66(1):608-11. | |
dc.identifier.issn | 0022-538X (Print) | |
dc.identifier.pmid | 1370094 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/38715 | |
dc.description.abstract | A CD4+ cytotoxic T-lymphocyte (CTL) clone, established from the peripheral blood of a human immunodeficiency virus (HIV)-seropositive donor, lysed autologous target cells that were infected with a recombinant vaccinia virus containing the gag gene of HIV type 1 and target cells pulsed with p24gag construct expressed in Escherichia coli. The recognition of the HLA-DQ-restricted epitope by this clone was further defined by using overlapping synthetic peptides. The epitope recognized by this CD4+ CTL clone (amino acids 140 to 148) overlaps with a CD8+ epitope and is highly conserved among all isolates of HIV type 1 that have been sequenced. Production and secretion of lymphokines such as interleukin-2 and interleukin-6 after specific antigenic stimulation were demonstrated by this gag-specific CD4+ CTL clone. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=1370094&dopt=Abstract">Link to Article in PubMed</a> | |
dc.subject | Amino Acid Sequence | |
dc.subject | CD4-Positive T-Lymphocytes | |
dc.subject | Clone Cells | |
dc.subject | Epitopes | |
dc.subject | HIV Core Protein p24 | |
dc.subject | Humans | |
dc.subject | Interleukin-2 | |
dc.subject | Interleukin-6 | |
dc.subject | Molecular Sequence Data | |
dc.subject | Life Sciences | |
dc.subject | Medicine and Health Sciences | |
dc.title | A CD4+ cytotoxic T-lymphocyte clone to a conserved epitope on human immunodeficiency virus type 1 p24: cytotoxic activity and secretion of interleukin-2 and interleukin-6 | |
dc.type | Journal Article | |
dc.source.journaltitle | Journal of virology | |
dc.source.volume | 66 | |
dc.source.issue | 1 | |
dc.identifier.legacyfulltext | https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2567&context=oapubs&unstamped=1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/oapubs/1568 | |
dc.identifier.contextkey | 798546 | |
refterms.dateFOA | 2022-08-23T16:36:07Z | |
html.description.abstract | <p>A CD4+ cytotoxic T-lymphocyte (CTL) clone, established from the peripheral blood of a human immunodeficiency virus (HIV)-seropositive donor, lysed autologous target cells that were infected with a recombinant vaccinia virus containing the gag gene of HIV type 1 and target cells pulsed with p24gag construct expressed in Escherichia coli. The recognition of the HLA-DQ-restricted epitope by this clone was further defined by using overlapping synthetic peptides. The epitope recognized by this CD4+ CTL clone (amino acids 140 to 148) overlaps with a CD8+ epitope and is highly conserved among all isolates of HIV type 1 that have been sequenced. Production and secretion of lymphokines such as interleukin-2 and interleukin-6 after specific antigenic stimulation were demonstrated by this gag-specific CD4+ CTL clone.</p> | |
dc.identifier.submissionpath | oapubs/1568 | |
dc.contributor.department | Center for Infectious Disease and Vaccine Research | |
dc.contributor.department | Department of Medicine, Division of Infectious Diseases and Immunology | |
dc.source.pages | 608-11 |