Requirement of a centrosomal activity for cell cycle progression through G1 into S phase
AuthorsHinchcliffe, Edward H.
Miller, Frederick J.
Khodjakov, Alexey L.
UMass Chan AffiliationsDepartment of Cell Biology
Document TypeJournal Article
Mitotic Spindle Apparatus
Medicine and Health Sciences
MetadataShow full item record
AbstractCentrosomes were microsurgically removed from BSC-1 African green monkey kidney cells before the completion of S phase. Karyoplasts (acentrosomal cells) entered and completed mitosis. However, postmitotic karyoplasts arrested before S phase, whereas adjacent control cells divided repeatedly. Postmitotic karyoplasts assembled a microtubule-organizing center containing gamma-tubulin and pericentrin, but did not regenerate centrioles. These observations reveal the existence of an activity associated with core centrosomal structures-distinct from elements of the microtubule-organizing center-that is required for the somatic cell cycle to progress through G1 into S phase. Once the cell is in S phase, these core structures are not needed for the G2-M phase transition.
SourceScience. 2001 Feb 23;291(5508):1547-50. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/38793
Related ResourcesLink to Article in PubMed