Nuclear accumulation of NFAT4 opposed by the JNK signal transduction pathway
| dc.contributor.author | Chow, Chi-Wing | |
| dc.contributor.author | Rincon, Mercedes | |
| dc.contributor.author | Cavanagh, Julie | |
| dc.contributor.author | Dickens, Martin | |
| dc.contributor.author | Davis, Roger J. | |
| dc.date | 2022-08-11T08:09:35.000 | |
| dc.date.accessioned | 2022-08-23T16:36:30Z | |
| dc.date.available | 2022-08-23T16:36:30Z | |
| dc.date.issued | 1997-12-31 | |
| dc.date.submitted | 2009-03-31 | |
| dc.identifier.citation | <p>Science. 1997 Nov 28;278(5343):1638-41.</p> | |
| dc.identifier.issn | 0036-8075 (Print) | |
| dc.identifier.doi | 10.1126/science.278.5343.1638 | |
| dc.identifier.pmid | 9374467 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/38801 | |
| dc.description.abstract | The nuclear factor of activated T cells (NFAT) group of transcription factors is retained in the cytoplasm of quiescent cells. NFAT activation is mediated in part by induced nuclear import. This process requires calcium-dependent dephosphorylation of NFAT caused by the phosphatase calcineurin. The c-Jun amino-terminal kinase (JNK) phosphorylates NFAT4 on two sites. Mutational removal of the JNK phosphorylation sites caused constitutive nuclear localization of NFAT4. In contrast, JNK activation in calcineurin-stimulated cells caused nuclear exclusion of NFAT4. These findings show that the nuclear accumulation of NFAT4 promoted by calcineurin is opposed by the JNK signal transduction pathway. | |
| dc.language.iso | en_US | |
| dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=9374467&dopt=Abstract">Link to Article in PubMed</a></p> | |
| dc.relation.url | https://doi.org/10.1126/science.278.5343.1638 | |
| dc.subject | Animals | |
| dc.subject | Binding Sites | |
| dc.subject | COS Cells | |
| dc.subject | Calcineurin | |
| dc.subject | Calcium-Calmodulin-Dependent Protein Kinases | |
| dc.subject | Cell Line | |
| dc.subject | Cell Nucleus | |
| dc.subject | Cyclosporine | |
| dc.subject | Cytoplasm | |
| dc.subject | DNA-Binding Proteins | |
| dc.subject | Humans | |
| dc.subject | JNK Mitogen-Activated Protein Kinases | |
| dc.subject | Jurkat Cells | |
| dc.subject | Mitogen-Activated Protein Kinase Kinases | |
| dc.subject | *Mitogen-Activated Protein Kinases | |
| dc.subject | Mutation | |
| dc.subject | NFATC Transcription Factors | |
| dc.subject | *Nuclear Proteins | |
| dc.subject | Phosphorylation | |
| dc.subject | Protein Kinases | |
| dc.subject | Recombinant Fusion Proteins | |
| dc.subject | *Signal Transduction | |
| dc.subject | T-Lymphocytes | |
| dc.subject | Transcription Factors | |
| dc.subject | Transcription, Genetic | |
| dc.subject | Life Sciences | |
| dc.subject | Medicine and Health Sciences | |
| dc.title | Nuclear accumulation of NFAT4 opposed by the JNK signal transduction pathway | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Science (New York, N.Y.) | |
| dc.source.volume | 278 | |
| dc.source.issue | 5343 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/oapubs/1645 | |
| dc.identifier.contextkey | 805466 | |
| html.description.abstract | <p>The nuclear factor of activated T cells (NFAT) group of transcription factors is retained in the cytoplasm of quiescent cells. NFAT activation is mediated in part by induced nuclear import. This process requires calcium-dependent dephosphorylation of NFAT caused by the phosphatase calcineurin. The c-Jun amino-terminal kinase (JNK) phosphorylates NFAT4 on two sites. Mutational removal of the JNK phosphorylation sites caused constitutive nuclear localization of NFAT4. In contrast, JNK activation in calcineurin-stimulated cells caused nuclear exclusion of NFAT4. These findings show that the nuclear accumulation of NFAT4 promoted by calcineurin is opposed by the JNK signal transduction pathway.</p> | |
| dc.identifier.submissionpath | oapubs/1645 | |
| dc.contributor.department | Howard Hughes Medical Institute and Program in Molecular Medicine | |
| dc.source.pages | 1638-41 |
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