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    Mobilization of peripheral blood progenitor cells by Betafectin PGG-Glucan alone and in combination with granulocyte colony-stimulating factor

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    Authors
    Patchen, Myra L.
    Liang, Jinsheng
    Vaudrain, Tracy
    Martin, Tracey
    Melican, David
    Zhong, Suju
    Stewart, F. Marc
    Quesenberry, Peter J.
    UMass Chan Affiliations
    Cancer Center
    Document Type
    Journal Article
    Publication Date
    1998-06-09
    Keywords
    Adjuvants, Immunologic
    Animals
    Bone Marrow Cells
    Cell Wall
    Cells, Cultured
    Colony-Forming Units Assay
    Drug Synergism
    Erythrocyte Count
    Female
    Glucans
    Granulocyte Colony-Stimulating Factor
    *Hematopoietic Stem Cell Transplantation
    Hematopoietic Stem Cells
    Leukocyte Count
    Male
    Mice
    Mice, Inbred C3H
    Platelet Count
    Saccharomyces cerevisiae
    Spleen
    *beta-Glucans
    Life Sciences
    Medicine and Health Sciences
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    Metadata
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    Link to Full Text
    https://doi.org/10.1002/stem.160208
    Abstract
    Betafectin PGG-Glucan, a novel beta-(1,6) branched beta-(1,3) glucan purified from the cell walls of Saccharomyces cerevisiae, has been shown to synergize with myeloid growth factors in vitro and to enhance hematopoietic recovery in myelosuppressed mice and primates. Here we report that PGG-Glucan is also capable of mobilizing peripheral blood progenitor cells (PBPC). PGG-Glucan (0.5 mg/kg to 16 mg/kg) was administered intravenously to C3H/HeN male mice and blood collected at times ranging from 30 min to seven days after injection. Based on granulocyte-macrophage colony-forming cell (GM-CFC) levels, peak mobilization occurred 30 min after a 2 mg/kg PGG-Glucan dose. At this time GM-CFC numbers in PGG-Glucan-treated mice were approximately fourfold greater than in saline-treated control mice. A second, smaller wave of GM-CFC mobilization (approximately twofold increase) also occurred on days 4 and 5 after PGG-Glucan treatment. Mobilization was not associated with the induction of alpha-chemokines, which have recently been reported to induce rapid progenitor cell mobilization. Competitive repopulation experiments performed in irradiated female C3H/HeN mice revealed that, at three months after transplantation, more male DNA was present in bone marrow, splenic, and thymic tissues from animals transplanted with cells obtained from mice 30 min after a 2 mg/kg PGG-Glucan dose than in tissues from animals transplanted with cells obtained from saline-treated mice. Additional experiments evaluated the mobilization effects of PGG-Glucan (2 mg/kg) administered to mice which had been pretreated for three consecutive days with G-CSF (125 microg/kg/day). When blood was collected 30 min after PGG-Glucan treatment, the number of GM-CFC mobilized in combination-treated mice was additive between the number mobilized in mice treated with G-CSF alone and the number mobilized in mice treated with PGG-Glucan alone. These studies demonstrate that: A) PGG-Glucan can rapidly mobilize PBPC; B) the kinetic pattern of PGG-Glucan-induced mobilization is different from that of the CSFs; C) the reconstitutional potential of PGG-Glucan mobilized cells is greater than that of steady-state PBPC, and D) PGG-Glucan can enhance G-CSF-mediated PBPC mobilization.
    Source

    Stem Cells. 1998;16(3):208-17.

    DOI
    10.1002/stem.160208
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/38805
    PubMed ID
    9617896
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    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1002/stem.160208
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