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Arrangement of Dam methylation sites (GATC) in the Escherichia coli chromosome

dc.contributor.authorBarras, Frederic
dc.contributor.authorMarinus, Martin G.
dc.date2022-08-11T08:09:35.000
dc.date.accessioned2022-08-23T16:36:50Z
dc.date.available2022-08-23T16:36:50Z
dc.date.issued1988-10-25
dc.date.submitted2009-04-02
dc.identifier.citationNucleic Acids Res. 1988 Oct 25;16(20):9821-38.
dc.identifier.issn0305-1048 (Print)
dc.identifier.pmid3054812
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38876
dc.description.abstractThe occurrence of GATC (Dam-recognition) sites in available E. coli DNA sequences (representing about 2% of the chromosome) has been determined by a simple numerical analysis. Our approach was to analyze the nucleotide composition of nine large sequenced DNA stretches ("cantles") in order to identify patterns of GATC distribution and to rationalize such patterns in biological/structural terms. The following observations were made: (i) In addition to oriC, GATC-rich regions are present in numerous locations. (ii) There is a wide variation in GATC frequency both between and within DNA cantles which led to the identification of a void-cluster pattern of GATC arrangement. The distance between two GATCs was never greater than 2 kb. (iii) GATC sites are found more frequently in translated regions than (in decreasing order) non-coding or non-translated regions. In particular, rRNA and tRNA encoding genes exhibit the lowest GATC content.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=3054812&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectBase Composition
dc.subjectBase Sequence
dc.subject*Chromosomes
dc.subjectCodon
dc.subjectDNA, Bacterial
dc.subjectEscherichia coli
dc.subject*Genes, Bacterial
dc.subjectGenetic Code
dc.subjectMethylation
dc.subjectNucleic Acid Conformation
dc.subjectOligonucleotides
dc.subjectOperon
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleArrangement of Dam methylation sites (GATC) in the Escherichia coli chromosome
dc.typeJournal Article
dc.source.journaltitleNucleic acids research
dc.source.volume16
dc.source.issue20
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2711&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1712
dc.identifier.contextkey808475
refterms.dateFOA2022-08-23T16:36:50Z
html.description.abstract<p>The occurrence of GATC (Dam-recognition) sites in available E. coli DNA sequences (representing about 2% of the chromosome) has been determined by a simple numerical analysis. Our approach was to analyze the nucleotide composition of nine large sequenced DNA stretches ("cantles") in order to identify patterns of GATC distribution and to rationalize such patterns in biological/structural terms. The following observations were made: (i) In addition to oriC, GATC-rich regions are present in numerous locations. (ii) There is a wide variation in GATC frequency both between and within DNA cantles which led to the identification of a void-cluster pattern of GATC arrangement. The distance between two GATCs was never greater than 2 kb. (iii) GATC sites are found more frequently in translated regions than (in decreasing order) non-coding or non-translated regions. In particular, rRNA and tRNA encoding genes exhibit the lowest GATC content.</p>
dc.identifier.submissionpathoapubs/1712
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages9821-38


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