JNK regulates lifespan in Caenorhabditis elegans by modulating nuclear translocation of forkhead transcription factor/DAF-16
Authors
Oh, Seung WookMukhopadhyay, Arnab
Svrzikapa, Nenad
Jiang, Feng
Davis, Roger J.
Tissenbaum, Heidi A.
Document Type
Journal ArticlePublication Date
2005-03-16Keywords
Active Transport, Cell NucleusAnimals
Animals, Genetically Modified
Base Sequence
Caenorhabditis elegans
Caenorhabditis elegans Proteins
DNA, Helminth
Genes, Helminth
Longevity
Mitogen-Activated Protein Kinase 8
Models, Biological
Phosphorylation
Signal Transduction
Transcription Factors
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
DAF-16/forkhead transcription factor, the downstream target of the insulin-like signaling in Caenorhabditis elegans, is indispensable for both lifespan regulation and stress resistance. Here, we demonstrate that c-Jun N-terminal kinase (JNK) is a positive regulator of DAF-16 in both processes. Our genetic analysis suggests that the JNK pathway acts in parallel with the insulin-like signaling pathway to regulate lifespan and both pathways converge onto DAF-16. We also show that JNK-1 directly interacts with and phosphorylates DAF-16. Moreover, in response to heat stress, JNK-1 promotes the translocation of DAF-16 into the nucleus. Our findings define an interaction between two well conserved proteins, JNK-1 and DAF-16, and provide a mechanism by which JNK regulates longevity and stress resistance.Source
Proc Natl Acad Sci U S A. 2005 Mar 22;102(12):4494-9. Epub 2005 Mar 14. Link to article on publisher's site
DOI
10.1073/pnas.0500749102Permanent Link to this Item
http://hdl.handle.net/20.500.14038/38925PubMed ID
15767565Related Resources
ae974a485f413a2113503eed53cd6c53
10.1073/pnas.0500749102