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    Isoproterenol stimulates rapid extrusion of sodium from isolated smooth muscle cells

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    Authors
    Moore, Edwin D. W.
    Fay, Fredric S.
    UMass Chan Affiliations
    Department of Physiology
    Document Type
    Journal Article
    Publication Date
    1993-09-01
    Keywords
    8-Bromo Cyclic Adenosine Monophosphate
    Animals
    Benzofurans
    Bufo marinus
    Carbachol
    Ethers, Cyclic
    Fluorescent Dyes
    Forskolin
    Isoproterenol
    Kinetics
    Microscopy, Fluorescence
    Muscle, Smooth
    Ouabain
    Pindolol
    Potassium
    Sodium
    Stomach
    Time Factors
    Life Sciences
    Medicine and Health Sciences
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    Link to Full Text
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC47287/
    Abstract
    beta-Agonists cause an inhibition of contractility and a transient stimulation of Na+/K+ pumping in smooth muscle cells of the stomach from the toad Bufo marinus. To determine if the stimulation of Na+/K+ pumping causes changes in intracellular [Na+] ([Na+]i) that might link Na+ pump stimulation to decrease Ca2+ availability for contraction, [Na+]i was measured in these cells with SBFI, a Na(+)-sensitive fluorescent indicator. Basal [Na+]i was 12.8 +/- 4.2 mM (n = 32) and was uniform throughout the cell. In response to isoproterenol, [Na+]i decreased an average of 7.1 +/- 1.1 mM in 3 sec. Since this decrease in [Na+]i could be completely blocked by inhibition of the Na+ pump, or by blockade of the beta-receptor, [Na+]i reduction is the result of occupation of the beta-receptor by isoproterenol and subsequent stimulation of the Na+ pump. 8-Bromoadenosine 3',5'-cyclic monophosphate and forskolin mimicked the effect of isoproterenol, indicating that the sequence of events linking beta-receptor occupation to Na+ pump stimulation most likely includes activation of adenylate cyclase, production of cAMP, and stimulation of cAMP-dependent protein kinase. The decrease in [Na+]i is sufficiently large and fast that it is expected to stimulate turnover of the Na+/Ca2+ exchanger in the Ca2+ extrusion mode, thereby accounting for the observed linkage between stimulation of the Na+/K+ pump and inhibition of contractility in response to beta-adrenergic agonists.
    Source

    Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8058-62.

    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/38986
    PubMed ID
    8367463
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