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dc.contributor.authorMatijasevic, Zdenka
dc.contributor.authorSekiguchi, Mutsuo
dc.contributor.authorLudlum, David B.
dc.date2022-08-11T08:09:36.000
dc.date.accessioned2022-08-23T16:37:21Z
dc.date.available2022-08-23T16:37:21Z
dc.date.issued1992-10-01
dc.date.submitted2009-04-02
dc.identifier.citation<p>Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9331-4.</p>
dc.identifier.issn0027-8424 (Print)
dc.identifier.pmid1409640
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38994
dc.description.abstractThe human carcinogen vinyl chloride is metabolized in the liver to reactive intermediates which form N2,3-ethenoguanine in DNA. N2,3-Ethenoguanine is known to cause G----A transitions during DNA replication in Escherichia coli, and its formation may be a carcinogenic event in higher organisms. To investigate the repair of N2,3-ethenoguanine, we have prepared an N2,3-etheno[14C]guanine-containing DNA substrate by nick-translating DNA with [14C]dGTP and modifying the product with chloroacetaldehyde. E. coli 3-methyladenine DNA glycosylase II, purified from cells which carry the plasmid pYN1000, releases N2,3-ethenoguanine from chloroacetaldehyde-modified DNA in a protein- and time-dependent manner. This finding widens the known substrate specificity of glycosylase II to include a modified base which may be associated with the carcinogenic process. Similar enzymatic activity in eukaryotic cell might protect them from exposure to metabolites of vinyl chloride.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=1409640&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC50120/
dc.subjectAcetaldehyde
dc.subjectCarbon Radioisotopes
dc.subjectChromatography, High Pressure Liquid
dc.subjectDNA
dc.subject*DNA Glycosylases
dc.subjectDNA Repair
dc.subjectDeoxyguanine Nucleotides
dc.subjectEscherichia coli
dc.subjectGuanine
dc.subjectKinetics
dc.subjectMethylnitrosourea
dc.subjectN-Glycosyl Hydrolases
dc.subjectTime Factors
dc.subjectTritium
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleRelease of N2,3-ethenoguanine from chloroacetaldehyde-treated DNA by Escherichia coli 3-methyladenine DNA glycosylase II
dc.typeJournal Article
dc.source.journaltitleProceedings of the National Academy of Sciences of the United States of America
dc.source.volume89
dc.source.issue19
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1820
dc.identifier.contextkey808586
html.description.abstract<p>The human carcinogen vinyl chloride is metabolized in the liver to reactive intermediates which form N2,3-ethenoguanine in DNA. N2,3-Ethenoguanine is known to cause G----A transitions during DNA replication in Escherichia coli, and its formation may be a carcinogenic event in higher organisms. To investigate the repair of N2,3-ethenoguanine, we have prepared an N2,3-etheno[14C]guanine-containing DNA substrate by nick-translating DNA with [14C]dGTP and modifying the product with chloroacetaldehyde. E. coli 3-methyladenine DNA glycosylase II, purified from cells which carry the plasmid pYN1000, releases N2,3-ethenoguanine from chloroacetaldehyde-modified DNA in a protein- and time-dependent manner. This finding widens the known substrate specificity of glycosylase II to include a modified base which may be associated with the carcinogenic process. Similar enzymatic activity in eukaryotic cell might protect them from exposure to metabolites of vinyl chloride.</p>
dc.identifier.submissionpathoapubs/1820
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages9331-4


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