Release of N2,3-ethenoguanine from chloroacetaldehyde-treated DNA by Escherichia coli 3-methyladenine DNA glycosylase II
dc.contributor.author | Matijasevic, Zdenka | |
dc.contributor.author | Sekiguchi, Mutsuo | |
dc.contributor.author | Ludlum, David B. | |
dc.date | 2022-08-11T08:09:36.000 | |
dc.date.accessioned | 2022-08-23T16:37:21Z | |
dc.date.available | 2022-08-23T16:37:21Z | |
dc.date.issued | 1992-10-01 | |
dc.date.submitted | 2009-04-02 | |
dc.identifier.citation | <p>Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9331-4.</p> | |
dc.identifier.issn | 0027-8424 (Print) | |
dc.identifier.pmid | 1409640 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/38994 | |
dc.description.abstract | The human carcinogen vinyl chloride is metabolized in the liver to reactive intermediates which form N2,3-ethenoguanine in DNA. N2,3-Ethenoguanine is known to cause G----A transitions during DNA replication in Escherichia coli, and its formation may be a carcinogenic event in higher organisms. To investigate the repair of N2,3-ethenoguanine, we have prepared an N2,3-etheno[14C]guanine-containing DNA substrate by nick-translating DNA with [14C]dGTP and modifying the product with chloroacetaldehyde. E. coli 3-methyladenine DNA glycosylase II, purified from cells which carry the plasmid pYN1000, releases N2,3-ethenoguanine from chloroacetaldehyde-modified DNA in a protein- and time-dependent manner. This finding widens the known substrate specificity of glycosylase II to include a modified base which may be associated with the carcinogenic process. Similar enzymatic activity in eukaryotic cell might protect them from exposure to metabolites of vinyl chloride. | |
dc.language.iso | en_US | |
dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=1409640&dopt=Abstract">Link to Article in PubMed</a></p> | |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC50120/ | |
dc.subject | Acetaldehyde | |
dc.subject | Carbon Radioisotopes | |
dc.subject | Chromatography, High Pressure Liquid | |
dc.subject | DNA | |
dc.subject | *DNA Glycosylases | |
dc.subject | DNA Repair | |
dc.subject | Deoxyguanine Nucleotides | |
dc.subject | Escherichia coli | |
dc.subject | Guanine | |
dc.subject | Kinetics | |
dc.subject | Methylnitrosourea | |
dc.subject | N-Glycosyl Hydrolases | |
dc.subject | Time Factors | |
dc.subject | Tritium | |
dc.subject | Life Sciences | |
dc.subject | Medicine and Health Sciences | |
dc.title | Release of N2,3-ethenoguanine from chloroacetaldehyde-treated DNA by Escherichia coli 3-methyladenine DNA glycosylase II | |
dc.type | Journal Article | |
dc.source.journaltitle | Proceedings of the National Academy of Sciences of the United States of America | |
dc.source.volume | 89 | |
dc.source.issue | 19 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/oapubs/1820 | |
dc.identifier.contextkey | 808586 | |
html.description.abstract | <p>The human carcinogen vinyl chloride is metabolized in the liver to reactive intermediates which form N2,3-ethenoguanine in DNA. N2,3-Ethenoguanine is known to cause G----A transitions during DNA replication in Escherichia coli, and its formation may be a carcinogenic event in higher organisms. To investigate the repair of N2,3-ethenoguanine, we have prepared an N2,3-etheno[14C]guanine-containing DNA substrate by nick-translating DNA with [14C]dGTP and modifying the product with chloroacetaldehyde. E. coli 3-methyladenine DNA glycosylase II, purified from cells which carry the plasmid pYN1000, releases N2,3-ethenoguanine from chloroacetaldehyde-modified DNA in a protein- and time-dependent manner. This finding widens the known substrate specificity of glycosylase II to include a modified base which may be associated with the carcinogenic process. Similar enzymatic activity in eukaryotic cell might protect them from exposure to metabolites of vinyl chloride.</p> | |
dc.identifier.submissionpath | oapubs/1820 | |
dc.contributor.department | Department of Biochemistry and Molecular Pharmacology | |
dc.source.pages | 9331-4 |