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dc.contributor.authorOkulicz, William C.
dc.contributor.authorBalsamo, Melissa
dc.contributor.authorTast, Janet
dc.date2022-08-11T08:09:39.000
dc.date.accessioned2022-08-23T16:38:51Z
dc.date.available2022-08-23T16:38:51Z
dc.date.issued1993-07-01
dc.date.submitted2008-03-26
dc.identifier.citation<p>Biol Reprod. 1993 Jul;49(1):24-32.</p>
dc.identifier.issn0006-3363 (Print)
dc.identifier.pmid8353187
dc.identifier.urihttp://hdl.handle.net/20.500.14038/39339
dc.description.abstractProgesterone (P) down-regulation of uterine estradiol (E) receptor (ER) appears to be a general mechanism by which P modulates E action in the uterus. Our present studies focus on the regulation of ER by P during the changeover from E to P dominance during artificial menstrual cycles in the rhesus monkey. Because of differential cell-type response and the cellular zonation of the primate uterus, we used immunohistochemical analysis in addition to biochemical assays to study the regulation of ER by P. Ki-67 immunoreactivity was used as an index of endometrial proliferation. We performed our analyses on Days 13 (peak of E), 14 (declining E and rising P), 17 (basal E and rising P), and 21 (basal E and peak P). ER immunoreactivity was present throughout the endometrium in luminal and glandular epithelia and stromal fibroblasts on Day 13. As E was withdrawn and P rose on Day 14 there were few distinct changes in ER staining in stromal and epithelial cells. On Day 17, immunoreactive staining showed a distinct reduction for stromal cells in all zones. Although luminal epithelial cells showed a decrease in immunoreactivity on Day 17, zones II, III, and IV retained positive staining for ER in glandular epithelia. ER staining in stromal cells on Day 21 was similar to the pattern observed on Day 17, whereas epithelial cells in zones I, II, and III showed a reduction in staining. Glandular epithelia in zone IV maintained strong positive staining for ER on Day 21.(ABSTRACT TRUNCATED AT 250 WORDS)
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8353187&dopt=Abstract ">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1095/biolreprod49.1.24
dc.subjectAnimals
dc.subjectDown-Regulation
dc.subjectEndometrium
dc.subjectFemale
dc.subjectImmunohistochemistry
dc.subjectKi-67 Antigen
dc.subjectMacaca mulatta
dc.subjectMenstrual Cycle
dc.subjectNeoplasm Proteins
dc.subjectNuclear Proteins
dc.subjectOvariectomy
dc.subjectProgesterone
dc.subjectReceptors, Estrogen
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleProgesterone regulation of endometrial estrogen receptor and cell proliferation during the late proliferative and secretory phase in artificial menstrual cycles in the rhesus monkey
dc.typeArticle
dc.source.journaltitleBiology of reproduction
dc.source.volume49
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/214
dc.identifier.contextkey472715
html.description.abstract<p>Progesterone (P) down-regulation of uterine estradiol (E) receptor (ER) appears to be a general mechanism by which P modulates E action in the uterus. Our present studies focus on the regulation of ER by P during the changeover from E to P dominance during artificial menstrual cycles in the rhesus monkey. Because of differential cell-type response and the cellular zonation of the primate uterus, we used immunohistochemical analysis in addition to biochemical assays to study the regulation of ER by P. Ki-67 immunoreactivity was used as an index of endometrial proliferation. We performed our analyses on Days 13 (peak of E), 14 (declining E and rising P), 17 (basal E and rising P), and 21 (basal E and peak P). ER immunoreactivity was present throughout the endometrium in luminal and glandular epithelia and stromal fibroblasts on Day 13. As E was withdrawn and P rose on Day 14 there were few distinct changes in ER staining in stromal and epithelial cells. On Day 17, immunoreactive staining showed a distinct reduction for stromal cells in all zones. Although luminal epithelial cells showed a decrease in immunoreactivity on Day 17, zones II, III, and IV retained positive staining for ER in glandular epithelia. ER staining in stromal cells on Day 21 was similar to the pattern observed on Day 17, whereas epithelial cells in zones I, II, and III showed a reduction in staining. Glandular epithelia in zone IV maintained strong positive staining for ER on Day 21.(ABSTRACT TRUNCATED AT 250 WORDS)</p>
dc.identifier.submissionpathoapubs/214
dc.contributor.departmentDepartment of Obstetrics and Gynecology
dc.source.pages24-32


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