Generation of protective T cell-independent antiviral antibody responses in SCID mice reconstituted with follicular or marginal zone B cells
Document Type
Journal ArticlePublication Date
2009-06-23Keywords
Acute DiseaseAdoptive Transfer
Animals
Antibodies, Viral
Antigens, T-Independent
B-Lymphocyte Subsets
Clone Cells
Immunoglobulin G
Immunoglobulin M
Immunophenotyping
Lymphoma, B-Cell, Marginal Zone
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, SCID
Polyomavirus Infections
Spleen
Survival Analysis
T-Lymphocyte Subsets
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
B cells generated in the bone marrow of adult mice enter the periphery as transitional B cells and subsequently differentiate into one of two phenotypically and functionally distinct subsets, marginal zone (MZ) or follicular (Fo) B cells. Recent reports indicate, however, that in response to environmental cues, such as lymphopenia, mature Fo B cells can change to display phenotypic markers characteristic of MZ B cells. Previously, we found that splenic B cells transferred to SCID mice responded to polyoma virus (PyV) infection with T cell-independent (TI) IgM and IgG secretion, reducing the viral load and protecting mice from the lethal effect of the infection. The contribution of MZ and Fo B cell subsets to this antiviral TI-2 response, however, has not been addressed. In this study, we show that both sort-purified MZ and Fo B cells generate protective TI Ab responses to PyV infection when transferred into SCID mice. Moreover, the transferred Fo B cells in the spleens of the PyV-infected SCID mice change phenotype, with many of them displaying MZ B cell characteristics. These findings demonstrate the plasticity of the B cell subsets in virus-infected hosts and show for the first time that B cells derived exclusively from Fo B cells can effectively function in antiviral TI-2 responses.Source
J Immunol. 2009 Jul 1;183(1):518-23. Link to article on publisher's site
DOI
10.4049/jimmunol.0900068Permanent Link to this Item
http://hdl.handle.net/20.500.14038/39362PubMed ID
19542462Related Resources
ae974a485f413a2113503eed53cd6c53
10.4049/jimmunol.0900068