We are upgrading the repository! A content freeze is in effect until December 6, 2024. New submissions or changes to existing items will not be allowed during this period. All content already published will remain publicly available for searching and downloading. Updates will be posted in the Website Upgrade 2024 FAQ in the sidebar Help menu. Reach out to escholarship@umassmed.edu with any questions.
Generation of protective T cell-independent antiviral antibody responses in SCID mice reconstituted with follicular or marginal zone B cells
Document Type
Journal ArticlePublication Date
2009-06-23Keywords
Acute DiseaseAdoptive Transfer
Animals
Antibodies, Viral
Antigens, T-Independent
B-Lymphocyte Subsets
Clone Cells
Immunoglobulin G
Immunoglobulin M
Immunophenotyping
Lymphoma, B-Cell, Marginal Zone
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, SCID
Polyomavirus Infections
Spleen
Survival Analysis
T-Lymphocyte Subsets
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
B cells generated in the bone marrow of adult mice enter the periphery as transitional B cells and subsequently differentiate into one of two phenotypically and functionally distinct subsets, marginal zone (MZ) or follicular (Fo) B cells. Recent reports indicate, however, that in response to environmental cues, such as lymphopenia, mature Fo B cells can change to display phenotypic markers characteristic of MZ B cells. Previously, we found that splenic B cells transferred to SCID mice responded to polyoma virus (PyV) infection with T cell-independent (TI) IgM and IgG secretion, reducing the viral load and protecting mice from the lethal effect of the infection. The contribution of MZ and Fo B cell subsets to this antiviral TI-2 response, however, has not been addressed. In this study, we show that both sort-purified MZ and Fo B cells generate protective TI Ab responses to PyV infection when transferred into SCID mice. Moreover, the transferred Fo B cells in the spleens of the PyV-infected SCID mice change phenotype, with many of them displaying MZ B cell characteristics. These findings demonstrate the plasticity of the B cell subsets in virus-infected hosts and show for the first time that B cells derived exclusively from Fo B cells can effectively function in antiviral TI-2 responses.Source
J Immunol. 2009 Jul 1;183(1):518-23. Link to article on publisher's site
DOI
10.4049/jimmunol.0900068Permanent Link to this Item
http://hdl.handle.net/20.500.14038/39362PubMed ID
19542462Related Resources
ae974a485f413a2113503eed53cd6c53
10.4049/jimmunol.0900068