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dc.contributor.authorGuay, Heath M.
dc.contributor.authorMishra, Rabinarayan
dc.contributor.authorGarcea, Robert L.
dc.contributor.authorWelsh, Raymond M.
dc.contributor.authorSzomolanyi-Tsuda, Eva
dc.date2022-08-11T08:09:39.000
dc.date.accessioned2022-08-23T16:38:57Z
dc.date.available2022-08-23T16:38:57Z
dc.date.issued2009-06-23
dc.date.submitted2010-04-01
dc.identifier.citation<p>J Immunol. 2009 Jul 1;183(1):518-23. <a href="http://dx.doi.org/10.4049/jimmunol.0900068">Link to article on publisher's site</a></p>
dc.identifier.issn0022-1767 (Linking)
dc.identifier.doi10.4049/jimmunol.0900068
dc.identifier.pmid19542462
dc.identifier.urihttp://hdl.handle.net/20.500.14038/39362
dc.description.abstractB cells generated in the bone marrow of adult mice enter the periphery as transitional B cells and subsequently differentiate into one of two phenotypically and functionally distinct subsets, marginal zone (MZ) or follicular (Fo) B cells. Recent reports indicate, however, that in response to environmental cues, such as lymphopenia, mature Fo B cells can change to display phenotypic markers characteristic of MZ B cells. Previously, we found that splenic B cells transferred to SCID mice responded to polyoma virus (PyV) infection with T cell-independent (TI) IgM and IgG secretion, reducing the viral load and protecting mice from the lethal effect of the infection. The contribution of MZ and Fo B cell subsets to this antiviral TI-2 response, however, has not been addressed. In this study, we show that both sort-purified MZ and Fo B cells generate protective TI Ab responses to PyV infection when transferred into SCID mice. Moreover, the transferred Fo B cells in the spleens of the PyV-infected SCID mice change phenotype, with many of them displaying MZ B cell characteristics. These findings demonstrate the plasticity of the B cell subsets in virus-infected hosts and show for the first time that B cells derived exclusively from Fo B cells can effectively function in antiviral TI-2 responses.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=19542462&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774789/
dc.subjectAcute Disease
dc.subjectAdoptive Transfer
dc.subjectAnimals
dc.subjectAntibodies, Viral
dc.subjectAntigens, T-Independent
dc.subjectB-Lymphocyte Subsets
dc.subjectClone Cells
dc.subjectImmunoglobulin G
dc.subjectImmunoglobulin M
dc.subjectImmunophenotyping
dc.subjectLymphoma, B-Cell, Marginal Zone
dc.subjectMice
dc.subjectMice, Inbred C57BL
dc.subjectMice, Knockout
dc.subjectMice, SCID
dc.subjectPolyomavirus Infections
dc.subjectSpleen
dc.subjectSurvival Analysis
dc.subjectT-Lymphocyte Subsets
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleGeneration of protective T cell-independent antiviral antibody responses in SCID mice reconstituted with follicular or marginal zone B cells
dc.typeJournal Article
dc.source.journaltitleJournal of immunology (Baltimore, Md. : 1950)
dc.source.volume183
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/2160
dc.identifier.contextkey1257937
html.description.abstract<p>B cells generated in the bone marrow of adult mice enter the periphery as transitional B cells and subsequently differentiate into one of two phenotypically and functionally distinct subsets, marginal zone (MZ) or follicular (Fo) B cells. Recent reports indicate, however, that in response to environmental cues, such as lymphopenia, mature Fo B cells can change to display phenotypic markers characteristic of MZ B cells. Previously, we found that splenic B cells transferred to SCID mice responded to polyoma virus (PyV) infection with T cell-independent (TI) IgM and IgG secretion, reducing the viral load and protecting mice from the lethal effect of the infection. The contribution of MZ and Fo B cell subsets to this antiviral TI-2 response, however, has not been addressed. In this study, we show that both sort-purified MZ and Fo B cells generate protective TI Ab responses to PyV infection when transferred into SCID mice. Moreover, the transferred Fo B cells in the spleens of the PyV-infected SCID mice change phenotype, with many of them displaying MZ B cell characteristics. These findings demonstrate the plasticity of the B cell subsets in virus-infected hosts and show for the first time that B cells derived exclusively from Fo B cells can effectively function in antiviral TI-2 responses.</p>
dc.identifier.submissionpathoapubs/2160
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.contributor.departmentDepartment of Pathology
dc.source.pages518-23


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