Generation of protective T cell-independent antiviral antibody responses in SCID mice reconstituted with follicular or marginal zone B cells
dc.contributor.author | Guay, Heath M. | |
dc.contributor.author | Mishra, Rabinarayan | |
dc.contributor.author | Garcea, Robert L. | |
dc.contributor.author | Welsh, Raymond M. | |
dc.contributor.author | Szomolanyi-Tsuda, Eva | |
dc.date | 2022-08-11T08:09:39.000 | |
dc.date.accessioned | 2022-08-23T16:38:57Z | |
dc.date.available | 2022-08-23T16:38:57Z | |
dc.date.issued | 2009-06-23 | |
dc.date.submitted | 2010-04-01 | |
dc.identifier.citation | <p>J Immunol. 2009 Jul 1;183(1):518-23. <a href="http://dx.doi.org/10.4049/jimmunol.0900068">Link to article on publisher's site</a></p> | |
dc.identifier.issn | 0022-1767 (Linking) | |
dc.identifier.doi | 10.4049/jimmunol.0900068 | |
dc.identifier.pmid | 19542462 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/39362 | |
dc.description.abstract | B cells generated in the bone marrow of adult mice enter the periphery as transitional B cells and subsequently differentiate into one of two phenotypically and functionally distinct subsets, marginal zone (MZ) or follicular (Fo) B cells. Recent reports indicate, however, that in response to environmental cues, such as lymphopenia, mature Fo B cells can change to display phenotypic markers characteristic of MZ B cells. Previously, we found that splenic B cells transferred to SCID mice responded to polyoma virus (PyV) infection with T cell-independent (TI) IgM and IgG secretion, reducing the viral load and protecting mice from the lethal effect of the infection. The contribution of MZ and Fo B cell subsets to this antiviral TI-2 response, however, has not been addressed. In this study, we show that both sort-purified MZ and Fo B cells generate protective TI Ab responses to PyV infection when transferred into SCID mice. Moreover, the transferred Fo B cells in the spleens of the PyV-infected SCID mice change phenotype, with many of them displaying MZ B cell characteristics. These findings demonstrate the plasticity of the B cell subsets in virus-infected hosts and show for the first time that B cells derived exclusively from Fo B cells can effectively function in antiviral TI-2 responses. | |
dc.language.iso | en_US | |
dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=19542462&dopt=Abstract">Link to Article in PubMed</a></p> | |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774789/ | |
dc.subject | Acute Disease | |
dc.subject | Adoptive Transfer | |
dc.subject | Animals | |
dc.subject | Antibodies, Viral | |
dc.subject | Antigens, T-Independent | |
dc.subject | B-Lymphocyte Subsets | |
dc.subject | Clone Cells | |
dc.subject | Immunoglobulin G | |
dc.subject | Immunoglobulin M | |
dc.subject | Immunophenotyping | |
dc.subject | Lymphoma, B-Cell, Marginal Zone | |
dc.subject | Mice | |
dc.subject | Mice, Inbred C57BL | |
dc.subject | Mice, Knockout | |
dc.subject | Mice, SCID | |
dc.subject | Polyomavirus Infections | |
dc.subject | Spleen | |
dc.subject | Survival Analysis | |
dc.subject | T-Lymphocyte Subsets | |
dc.subject | Life Sciences | |
dc.subject | Medicine and Health Sciences | |
dc.title | Generation of protective T cell-independent antiviral antibody responses in SCID mice reconstituted with follicular or marginal zone B cells | |
dc.type | Journal Article | |
dc.source.journaltitle | Journal of immunology (Baltimore, Md. : 1950) | |
dc.source.volume | 183 | |
dc.source.issue | 1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/oapubs/2160 | |
dc.identifier.contextkey | 1257937 | |
html.description.abstract | <p>B cells generated in the bone marrow of adult mice enter the periphery as transitional B cells and subsequently differentiate into one of two phenotypically and functionally distinct subsets, marginal zone (MZ) or follicular (Fo) B cells. Recent reports indicate, however, that in response to environmental cues, such as lymphopenia, mature Fo B cells can change to display phenotypic markers characteristic of MZ B cells. Previously, we found that splenic B cells transferred to SCID mice responded to polyoma virus (PyV) infection with T cell-independent (TI) IgM and IgG secretion, reducing the viral load and protecting mice from the lethal effect of the infection. The contribution of MZ and Fo B cell subsets to this antiviral TI-2 response, however, has not been addressed. In this study, we show that both sort-purified MZ and Fo B cells generate protective TI Ab responses to PyV infection when transferred into SCID mice. Moreover, the transferred Fo B cells in the spleens of the PyV-infected SCID mice change phenotype, with many of them displaying MZ B cell characteristics. These findings demonstrate the plasticity of the B cell subsets in virus-infected hosts and show for the first time that B cells derived exclusively from Fo B cells can effectively function in antiviral TI-2 responses.</p> | |
dc.identifier.submissionpath | oapubs/2160 | |
dc.contributor.department | Department of Molecular Genetics and Microbiology | |
dc.contributor.department | Department of Pathology | |
dc.source.pages | 518-23 |