Endodomain diversity in the Drosophila Dscam and its roles in neuronal morphogenesis
Student Authors
Shun-Jen (Jacob) S. YangAcademic Program
NeuroscienceUMass Chan Affiliations
Graduate School of Biomedical Sciences, Neuroscience ProgramLee Lab
Neurobiology
Document Type
Journal ArticlePublication Date
2009-02-13Keywords
Amino Acid MotifsAmino Acid Sequence
Animals
Animals, Genetically Modified
Cell Adhesion Molecules
Drosophila
Drosophila Proteins
Gene Targeting
Molecular Sequence Data
Morphogenesis
Neurogenesis
Neurons
Protein Structure, Tertiary
Neuroscience and Neurobiology
Metadata
Show full item recordAbstract
Drosophila Down syndrome cell adhesion molecule (Dscam) can be variably spliced to encode 152,064 distinct single-pass transmembrane proteins. In addition to 19,008 possible ectodomains and two alternative transmembrane segments, it may carry endodomains containing or lacking exons 19 and 23. Here, we determine the role of Dscam endodomain diversity in neural development. Dscam with full-length endodomain is largely restricted to embryogenesis. In contrast, most Dscams lack exons 19 and 23 at postembryonic stages. As implicated from the expression patterns, removal of Dscam exon 19-containing variants disrupts wiring of embryonic neurons while silencing of Dscam transcripts lacking exon 19 or exon 23 effectively blocks postembryonic neuronal morphogenesis. Furthermore, compared with exon 19-containing Dscam, transgenic Dscam without exon 19 is more efficiently targeted to neurites and more potently suppresses axon bifurcation in Dscam mutant neurons. In sum, Dscam with or without exon 19 in its endodomain is used to govern different stage-specific neuronal morphogenetic processes, possibly due to differences in protein targeting.Source
J Neurosci. 2009 Feb 11;29(6):1904-14. Link to article on publisher's siteDOI
10.1523/JNEUROSCI.5743-08.2009Permanent Link to this Item
http://hdl.handle.net/20.500.14038/39366PubMed ID
19211897Notes
Co-author Jacob S. Yang is a student in the Neuroscience program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.
Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1523/JNEUROSCI.5743-08.2009