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dc.contributor.authorWu, J. T.
dc.date2022-08-11T08:09:39.000
dc.date.accessioned2022-08-23T16:39:00Z
dc.date.available2022-08-23T16:39:00Z
dc.date.issued1988-12-01
dc.date.submitted2008-03-26
dc.identifier.citation<p>Biol Reprod. 1988 Dec;39(5):1021-6.</p>
dc.identifier.issn0006-3363 (Print)
dc.identifier.pmid3219374
dc.identifier.urihttp://hdl.handle.net/20.500.14038/39372
dc.description.abstractThe rate of estrone (E1)----estradiol-17 beta (E2) or E2----E1 conversion catalyzed by 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) activity was determined for each mouse embryo in modified F-10 medium containing 0.95 microM 3H-E1 or 3H-E2. During delayed implantation, the E1----E2 conversion rate was decreased (p less than 0.005) from 5.69 +/- 0.34 fmol/h/blastocyst on Day 5 to 3.50 +/- 0.46 fmol/h/blastocyst on Day 9, whereas E2----E1 was increased (p less than 0.005) from 7.44 +/- 1.08 to 18.60 +/- 2.04 fmol/h/blastocyst. After estrogen injection, the Day 9 implanting blastocyst showed an increase (p less than 0.005) in E1----E2 conversion to 9.05 +/- 0.64 fmol/h/blastocyst but a slight, insignificant decrease in E2----E1 conversion to 14.2 +/- 1.82 fmol/h/blastocyst. A similar trend was also observed in Day 5 implanting blastocysts when compared to Day 5 delayed blastocysts. Thus, 17 beta-HSD activity in delayed blastocysts favors E2----E1 over E1----E2 conversion in a ratio of 5:1. After estrogen induction of implantation, the E1----E2 conversion rate is stimulated and the ratio of E2----E1 to E1----E2 rate is decreased to 1.5:1. The results suggest that 17 beta-HSD activity may be involved in blastocyst implantation.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=3219374&dopt=Abstract ">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1095/biolreprod39.5.1021
dc.subject17-Hydroxysteroid Dehydrogenases
dc.subjectAnimals
dc.subjectBlastocyst
dc.subjectCulture Techniques
dc.subject*Embryo Implantation
dc.subject*Embryo Implantation, Delayed
dc.subjectFemale
dc.subjectMice
dc.subjectPregnancy
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleChanges in the 17 beta-hydroxysteroid dehydrogenase activity of mouse blastocysts during delayed implantation
dc.typeJournal Article
dc.source.journaltitleBiology of reproduction
dc.source.volume39
dc.source.issue5
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/217
dc.identifier.contextkey472718
html.description.abstract<p>The rate of estrone (E1)----estradiol-17 beta (E2) or E2----E1 conversion catalyzed by 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) activity was determined for each mouse embryo in modified F-10 medium containing 0.95 microM 3H-E1 or 3H-E2. During delayed implantation, the E1----E2 conversion rate was decreased (p less than 0.005) from 5.69 +/- 0.34 fmol/h/blastocyst on Day 5 to 3.50 +/- 0.46 fmol/h/blastocyst on Day 9, whereas E2----E1 was increased (p less than 0.005) from 7.44 +/- 1.08 to 18.60 +/- 2.04 fmol/h/blastocyst. After estrogen injection, the Day 9 implanting blastocyst showed an increase (p less than 0.005) in E1----E2 conversion to 9.05 +/- 0.64 fmol/h/blastocyst but a slight, insignificant decrease in E2----E1 conversion to 14.2 +/- 1.82 fmol/h/blastocyst. A similar trend was also observed in Day 5 implanting blastocysts when compared to Day 5 delayed blastocysts. Thus, 17 beta-HSD activity in delayed blastocysts favors E2----E1 over E1----E2 conversion in a ratio of 5:1. After estrogen induction of implantation, the E1----E2 conversion rate is stimulated and the ratio of E2----E1 to E1----E2 rate is decreased to 1.5:1. The results suggest that 17 beta-HSD activity may be involved in blastocyst implantation.</p>
dc.identifier.submissionpathoapubs/217
dc.contributor.departmentDepartment of Obstetrics and Gynecology
dc.source.pages1021-6


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