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dc.contributor.authorLin, Andrew C.
dc.contributor.authorTan, Chin Lik
dc.contributor.authorLin, Chien-Ling
dc.contributor.authorStrochlic, Laure
dc.contributor.authorHuang, Yi-Shuian
dc.contributor.authorRichter, Joel D.
dc.contributor.authorHolt, Christine E.
dc.date2022-08-11T08:09:40.000
dc.date.accessioned2022-08-23T16:39:28Z
dc.date.available2022-08-23T16:39:28Z
dc.date.issued2009-03-02
dc.date.submitted2012-03-22
dc.identifier.citationNeural Dev. 2009 Mar 2;4:8. <a href="http://dx.doi.org/10.1186/1749-8104-4-8" target="_blank">Link to article on publisher's site</a> 2009 Lin et al.; licensee BioMed Central Ltd.
dc.identifier.issn1749-8104 (Linking)
dc.identifier.doi10.1186/1749-8104-4-8
dc.identifier.pmid19254368
dc.identifier.pmid19254368
dc.identifier.urihttp://hdl.handle.net/20.500.14038/39479
dc.description.abstractBACKGROUND: Translation in axons is required for growth cone chemotropic responses to many guidance cues. Although locally synthesized proteins are beginning to be identified, how specific mRNAs are selected for translation remains unclear. Control of poly(A) tail length by cytoplasmic polyadenylation element (CPE) binding protein 1 (CPEB1) is a conserved mechanism for mRNA-specific translational regulation that could be involved in regulating translation in axons. RESULTS: We show that cytoplasmic polyadenylation is required in Xenopus retinal ganglion cell (RGC) growth cones for translation-dependent, but not translation-independent, chemotropic responses in vitro, and that inhibition of CPE binding through dominant-negative interference severely reduces axon outgrowth in vivo. CPEB1 mRNA transcripts are present at low levels in RGCs but, surprisingly, CPEB1 protein was not detected in eye or brain tissue, and CPEB1 loss-of-function does not affect chemotropic responses or pathfinding in vivo. UV cross-linking experiments suggest that CPE-binding proteins other than CPEB1 in the retina regulate retinal axon development. CONCLUSION: These results indicate that cytoplasmic polyadenylation and CPE-mediated translational regulation are involved in retinal axon development, but that CPEB1 may not be the key regulator of polyadenylation in the developing retina.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=19254368&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1186/1749-8104-4-8
dc.rights© 2009 Lin et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.subjectAnimals
dc.subjectAxons
dc.subjectBase Sequence
dc.subjectCytoplasm
dc.subjectFluorescent Antibody Technique
dc.subjectGene Expression Regulation
dc.subjectIn Situ Hybridization
dc.subjectMolecular Sequence Data
dc.subjectPlasmids
dc.subjectPolyadenylation
dc.subjectProtein Biosynthesis
dc.subjectRNA, Messenger
dc.subjectRetinal Ganglion Cells
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectTranscription Factors
dc.subjectXenopus
dc.subjectXenopus Proteins
dc.subjectmRNA Cleavage and Polyadenylation Factors
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectNeuroscience and Neurobiology
dc.titleCytoplasmic polyadenylation and cytoplasmic polyadenylation element-dependent mRNA regulation are involved in Xenopus retinal axon development
dc.typeJournal Article
dc.source.journaltitleNeural development
dc.source.volume4
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=3273&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/2274
dc.identifier.contextkey2691113
refterms.dateFOA2022-08-23T16:39:28Z
html.description.abstract<p>BACKGROUND: Translation in axons is required for growth cone chemotropic responses to many guidance cues. Although locally synthesized proteins are beginning to be identified, how specific mRNAs are selected for translation remains unclear. Control of poly(A) tail length by cytoplasmic polyadenylation element (CPE) binding protein 1 (CPEB1) is a conserved mechanism for mRNA-specific translational regulation that could be involved in regulating translation in axons.</p> <p>RESULTS: We show that cytoplasmic polyadenylation is required in Xenopus retinal ganglion cell (RGC) growth cones for translation-dependent, but not translation-independent, chemotropic responses in vitro, and that inhibition of CPE binding through dominant-negative interference severely reduces axon outgrowth in vivo. CPEB1 mRNA transcripts are present at low levels in RGCs but, surprisingly, CPEB1 protein was not detected in eye or brain tissue, and CPEB1 loss-of-function does not affect chemotropic responses or pathfinding in vivo. UV cross-linking experiments suggest that CPE-binding proteins other than CPEB1 in the retina regulate retinal axon development.</p> <p>CONCLUSION: These results indicate that cytoplasmic polyadenylation and CPE-mediated translational regulation are involved in retinal axon development, but that CPEB1 may not be the key regulator of polyadenylation in the developing retina.</p>
dc.identifier.submissionpathoapubs/2274
dc.contributor.departmentProgram in Molecular Medicine
dc.source.pages8


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