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dc.contributor.authorRoy, Christian K.
dc.contributor.authorOlson, Sara
dc.contributor.authorGraveley, Brenton R.
dc.contributor.authorZamore, Phillip D.
dc.contributor.authorMoore, Melissa J.
dc.date2022-08-11T08:09:42.000
dc.date.accessioned2022-08-23T16:40:48Z
dc.date.available2022-08-23T16:40:48Z
dc.date.issued2015-04-13
dc.date.submitted2015-09-02
dc.identifier.citationElife. 2015 Apr 13;4:e03700. doi: 10.7554/eLife.03700. <a href="http://dx.doi.org/10.7554/eLife.03700">Link to article on publisher's site</a>
dc.identifier.issn2050-084X (Linking)
dc.identifier.doi10.7554/eLife.03700
dc.identifier.pmid25866926
dc.identifier.urihttp://hdl.handle.net/20.500.14038/39754
dc.description.abstractMany RNAs, including pre-mRNAs and long non-coding RNAs, can be thousands of nucleotides long and undergo complex post-transcriptional processing. Multiple sites of alternative splicing within a single gene exponentially increase the number of possible spliced isoforms, with most human genes currently estimated to express at least ten. To understand the mechanisms underlying these complex isoform expression patterns, methods are needed that faithfully maintain long-range exon connectivity information in individual RNA molecules. In this study, we describe SeqZip, a methodology that uses RNA-templated DNA-DNA ligation to retain and compress connectivity between distant sequences within single RNA molecules. Using this assay, we test proposed coordination between distant sites of alternative exon utilization in mouse Fn1, and we characterize the extraordinary exon diversity of Drosophila melanogaster Dscam1.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=25866926&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.7554/eLife.03700
dc.rightsCopyright © 2015, Roy et al. This article is distributed under the terms of the <a href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a>, which permits unrestricted use and redistribution provided that the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectD. melanogaster
dc.subjectDscam1
dc.subjectRNA-templated
dc.subjectalternative splicing
dc.subjectchromosomes
dc.subjectfibronectin
dc.subjectgenes
dc.subjectisoform
dc.subjectligation
dc.subjectmouse
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectGenetics and Genomics
dc.titleAssessing long-distance RNA sequence connectivity via RNA-templated DNA-DNA ligation
dc.typeJournal Article
dc.source.journaltitleeLife
dc.source.volume4
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=3554&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/2550
dc.identifier.contextkey7548580
refterms.dateFOA2022-08-23T16:40:48Z
html.description.abstract<p>Many RNAs, including pre-mRNAs and long non-coding RNAs, can be thousands of nucleotides long and undergo complex post-transcriptional processing. Multiple sites of alternative splicing within a single gene exponentially increase the number of possible spliced isoforms, with most human genes currently estimated to express at least ten. To understand the mechanisms underlying these complex isoform expression patterns, methods are needed that faithfully maintain long-range exon connectivity information in individual RNA molecules. In this study, we describe SeqZip, a methodology that uses RNA-templated DNA-DNA ligation to retain and compress connectivity between distant sequences within single RNA molecules. Using this assay, we test proposed coordination between distant sites of alternative exon utilization in mouse Fn1, and we characterize the extraordinary exon diversity of Drosophila melanogaster Dscam1.</p>
dc.identifier.submissionpathoapubs/2550
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.contributor.departmentRNA Therapeutics Institute
dc.source.pagese03700


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Copyright © 2015, Roy et al. This article is distributed under the terms of the <a href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a>, which permits unrestricted use and redistribution provided that the original author and source are credited.
Except where otherwise noted, this item's license is described as Copyright © 2015, Roy et al. This article is distributed under the terms of the <a href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a>, which permits unrestricted use and redistribution provided that the original author and source are credited.