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dc.contributor.authorHe, Lian
dc.contributor.authorZhang, Yuanwei
dc.contributor.authorMa, Guolin
dc.contributor.authorTan, Peng
dc.contributor.authorLi, Zhan Jun
dc.contributor.authorZhang, Shengbing
dc.contributor.authorWu, Xiang
dc.contributor.authorJing, Ji
dc.contributor.authorFang, Shaohai
dc.contributor.authorZhou, Lijuan
dc.contributor.authorWang, Youjun
dc.contributor.authorHuang, Yun
dc.contributor.authorHogan, Patrick
dc.contributor.authorHan, Gang
dc.contributor.authorZhou, Yubin
dc.date2022-08-11T08:09:44.000
dc.date.accessioned2022-08-23T16:41:15Z
dc.date.available2022-08-23T16:41:15Z
dc.date.issued2015-12-08
dc.date.submitted2015-12-23
dc.identifier.citationElife. 2015 Dec 8;4. pii: e10024. doi: 10.7554/eLife.10024. <a href="http://dx.doi.org/10.7554/eLife.10024">Link to article on publisher's site</a>
dc.identifier.issn2050-084X (Linking)
dc.identifier.doi10.7554/eLife.10024
dc.identifier.pmid26646180
dc.identifier.urihttp://hdl.handle.net/20.500.14038/39848
dc.description.abstractThe application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed "Opto-CRAC") that selectively and remotely controls Ca2+ oscillations and Ca2+-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca2+-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded "photoactivatable adjuvant" to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote control of Ca2+-modulated activities with tailored function.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=26646180&dopt=Abstract">Link to Article in PubMed</a>
dc.rights<p>© 2015, He et al. This article is distributed under the terms of the<a href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a>, which permits unrestricted use and redistribution provided that the original author and source are credited.</p>
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCalcium signaling
dc.subjectImmune response
dc.subjectNanoparticles
dc.subjectNear infrared
dc.subjectOptogenetics
dc.subjectSTIM1
dc.subjectbiochemistry
dc.subjectcell biology
dc.subjecthuman
dc.subjectmouse
dc.subjectBiochemistry
dc.subjectCell Biology
dc.subjectGenetics
dc.titleNear-infrared photoactivatable control of Ca signaling and optogenetic immunomodulation
dc.typeJournal Article
dc.source.journaltitleeLife
dc.source.volume4
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=3649&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/2645
dc.identifier.contextkey7972025
refterms.dateFOA2022-08-23T16:41:16Z
html.description.abstract<p>The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed "Opto-CRAC") that selectively and remotely controls Ca2+ oscillations and Ca2+-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca2+-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded "photoactivatable adjuvant" to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote control of Ca2+-modulated activities with tailored function.</p>
dc.identifier.submissionpathoapubs/2645
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology


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<p>© 2015, He et al. This article is distributed under the terms of the<a href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a>, which permits unrestricted use and redistribution provided that the original author and source are credited.</p>
Except where otherwise noted, this item's license is described as <p>© 2015, He et al. This article is distributed under the terms of the<a href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a>, which permits unrestricted use and redistribution provided that the original author and source are credited.</p>