Near-infrared photoactivatable control of Ca signaling and optogenetic immunomodulation
dc.contributor.author | He, Lian | |
dc.contributor.author | Zhang, Yuanwei | |
dc.contributor.author | Ma, Guolin | |
dc.contributor.author | Tan, Peng | |
dc.contributor.author | Li, Zhan Jun | |
dc.contributor.author | Zhang, Shengbing | |
dc.contributor.author | Wu, Xiang | |
dc.contributor.author | Jing, Ji | |
dc.contributor.author | Fang, Shaohai | |
dc.contributor.author | Zhou, Lijuan | |
dc.contributor.author | Wang, Youjun | |
dc.contributor.author | Huang, Yun | |
dc.contributor.author | Hogan, Patrick | |
dc.contributor.author | Han, Gang | |
dc.contributor.author | Zhou, Yubin | |
dc.date | 2022-08-11T08:09:44.000 | |
dc.date.accessioned | 2022-08-23T16:41:15Z | |
dc.date.available | 2022-08-23T16:41:15Z | |
dc.date.issued | 2015-12-08 | |
dc.date.submitted | 2015-12-23 | |
dc.identifier.citation | Elife. 2015 Dec 8;4. pii: e10024. doi: 10.7554/eLife.10024. <a href="http://dx.doi.org/10.7554/eLife.10024">Link to article on publisher's site</a> | |
dc.identifier.issn | 2050-084X (Linking) | |
dc.identifier.doi | 10.7554/eLife.10024 | |
dc.identifier.pmid | 26646180 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/39848 | |
dc.description.abstract | The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed "Opto-CRAC") that selectively and remotely controls Ca2+ oscillations and Ca2+-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca2+-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded "photoactivatable adjuvant" to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote control of Ca2+-modulated activities with tailored function. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=26646180&dopt=Abstract">Link to Article in PubMed</a> | |
dc.rights | <p>© 2015, He et al. This article is distributed under the terms of the<a href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a>, which permits unrestricted use and redistribution provided that the original author and source are credited.</p> | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Calcium signaling | |
dc.subject | Immune response | |
dc.subject | Nanoparticles | |
dc.subject | Near infrared | |
dc.subject | Optogenetics | |
dc.subject | STIM1 | |
dc.subject | biochemistry | |
dc.subject | cell biology | |
dc.subject | human | |
dc.subject | mouse | |
dc.subject | Biochemistry | |
dc.subject | Cell Biology | |
dc.subject | Genetics | |
dc.title | Near-infrared photoactivatable control of Ca signaling and optogenetic immunomodulation | |
dc.type | Journal Article | |
dc.source.journaltitle | eLife | |
dc.source.volume | 4 | |
dc.identifier.legacyfulltext | https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=3649&context=oapubs&unstamped=1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/oapubs/2645 | |
dc.identifier.contextkey | 7972025 | |
refterms.dateFOA | 2022-08-23T16:41:16Z | |
html.description.abstract | <p>The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed "Opto-CRAC") that selectively and remotely controls Ca2+ oscillations and Ca2+-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca2+-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded "photoactivatable adjuvant" to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote control of Ca2+-modulated activities with tailored function.</p> | |
dc.identifier.submissionpath | oapubs/2645 | |
dc.contributor.department | Department of Biochemistry and Molecular Pharmacology |