Positioning of AMPA Receptor-Containing Endosomes Regulates Synapse Architecture
AuthorsEsteves da Silva, Marta
Wierenga, Corette J.
Kapitein, Lukas C.
Hoogenraad, Casper C.
Student AuthorsSukhee Cho
UMass Chan AffiliationsGraduate School of Biomedical Sciences, Neuroscience Program
Department of Psychiatry
Brudnick Neuropsychiatric Research Institute
Document TypeJournal Article
Molecular and Cellular Neuroscience
MetadataShow full item record
AbstractLateral diffusion in the membrane and endosomal trafficking both contribute to the addition and removal of AMPA receptors (AMPARs) at postsynaptic sites. However, the spatial coordination between these mechanisms has remained unclear, because little is known about the dynamics of AMPAR-containing endosomes. In addition, how the positioning of AMPAR-containing endosomes affects synapse organization and functioning has never been directly explored. Here, we used live-cell imaging in hippocampal neuron cultures to show that intracellular AMPARs are transported in Rab11-positive recycling endosomes, which frequently enter dendritic spines and depend on the microtubule and actin cytoskeleton. By using chemically induced dimerization systems to recruit kinesin (KIF1C) or myosin (MyosinV/VI) motors to Rab11-positive recycling endosomes, we controlled their trafficking and found that induced removal of recycling endosomes from spines decreases surface AMPAR expression and PSD-95 clusters at synapses. Our data suggest a mechanistic link between endosome positioning and postsynaptic structure and composition.
SourceCell Rep. 2015 Nov 3;13(5):933-43. doi: 10.1016/j.celrep.2015.09.062. Epub 2015 Oct 22. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/39871
Co-author Sukhee Cho is a doctoral student in the Neuroscience Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.
Related ResourcesLink to Article in PubMed
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/