CpG Improves Influenza Vaccine Efficacy in Young Adult but Not Aged Mice
UMass Chan Affiliations
Division of Infectious Diseases and Immunology, Department of MedicineDocument Type
Journal ArticlePublication Date
2016-03-02Keywords
Immunology of Infectious DiseaseImmunoprophylaxis and Therapy
Influenza Humans
Influenza Virus Vaccines
Virus Diseases
Metadata
Show full item recordAbstract
Several studies have shown a reduced efficacy of influenza vaccines in the elderly compared to young adults. In this study, we evaluated the immunogenicity and protective efficacy of a commercially available inactivated influenza vaccine (Fluzone(R)) in young adult and aged mice. C57/BL6 mice were administered a single or double immunization of Fluzone(R) with or without CpG and challenged intranasally with H1N1 A/California/09 virus. A double immunization of Fluzone(R) adjuvanted with CpG elicited the highest level of protection in young adult mice which was associated with increases in influenza specific IgG, elevated HAI titres, reduced viral titres and lung inflammation. In contrast, the vaccine schedule which provided fully protective immunity in young adult mice conferred limited protection in aged mice. Antigen presenting cells from aged mice were found to be less responsive to in vitro stimulation by Fluzone and CpG which may partially explain this result. Our data are supportive of studies that have shown limited effectiveness of influenza vaccines in the elderly and provide important information relevant to the design of more immunogenic vaccines in this age group.Source
PLoS One. 2016 Mar 2;11(3):e0150425. doi: 10.1371/journal.pone.0150425. eCollection 2016. Link to article on publisher's siteDOI
10.1371/journal.pone.0150425Permanent Link to this Item
http://hdl.handle.net/20.500.14038/39966PubMed ID
26934728Related Resources
Link to Article in PubMedRights
Copyright: © 2016 Ramirez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Distribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0150425
Scopus Count
Collections
Except where otherwise noted, this item's license is described as Copyright: © 2016 Ramirez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</p>