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dc.contributor.authorDing, Baojin
dc.contributor.authorCave, John W.
dc.contributor.authorDobner, Paul R
dc.contributor.authorMullikin-Kilpatrick, Debra
dc.contributor.authorBartzokis, Marina
dc.contributor.authorZhu, Hong
dc.contributor.authorChow, Chi-Wing
dc.contributor.authorGronostajski, Richard M.
dc.contributor.authorKilpatrick, Daniel Lee
dc.date2022-08-11T08:09:45.000
dc.date.accessioned2022-08-23T16:42:11Z
dc.date.available2022-08-23T16:42:11Z
dc.date.issued2016-05-01
dc.date.submitted2016-08-16
dc.identifier.citation<p>Mol Biol Cell. 2016 May 1;27(9):1488-99. doi: 10.1091/mbc.E15-07-0476. Epub 2016 Mar 3. <a href="http://dx.doi.org/10.1091/mbc.E15-07-0476">Link to article on publisher's site</a></p>
dc.identifier.issn1059-1524 (Linking)
dc.identifier.doi10.1091/mbc.E15-07-0476
dc.identifier.pmid26941328
dc.identifier.urihttp://hdl.handle.net/20.500.14038/40040
dc.description.abstractNuclear Factor One (NFI) transcription factors regulate temporal gene expression required for dendritogenesis and synaptogenesis via delayed occupancy of target promoters in developing cerebellar granule neurons (CGNs). Mechanisms that promote NFI temporal occupancy have not been previously defined. We show here that the transcription factor ETV1 directly binds to and is required for expression and NFI occupancy of a cohort of NFI-dependent genes in CGNs maturing in vivo. Expression of ETV1 is low in early postnatal cerebellum and increases with maturation, mirroring NFI temporal occupancy of coregulated target genes. Precocious expression of ETV1 in mouse CGNs accelerated onset of expression and NFI temporal occupancy of late target genes and enhanced Map2(+) neurite outgrowth. ETV1 also activated expression and NFI occupancy of the Etv1 gene itself, and this autoregulatory loop preceded ETV1 binding and activation of other coregulated target genes in vivo. These findings suggest a potential model in which ETV1 activates NFI temporal binding to a subset of late-expressed genes in a stepwise manner by initial positive feedback regulation of the Etv1 gene itself followed by activation of downstream coregulated targets as ETV1 expression increases. Sequential transcription factor autoregulation and subsequent binding to downstream promoters may provide an intrinsic developmental timer for dendrite/synapse gene expression.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=26941328&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright © 2016 Ding et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/
dc.subjectCell Biology
dc.subjectMolecular Biology
dc.subjectNeuroscience and Neurobiology
dc.titleReciprocal autoregulation by NFI occupancy and ETV1 promotes the developmental expression of dendrite-synapse genes in cerebellar granule neurons
dc.typeJournal Article
dc.source.journaltitleMolecular biology of the cell
dc.source.volume27
dc.source.issue9
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=3851&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/2846
dc.identifier.contextkey8985382
refterms.dateFOA2022-08-23T16:42:11Z
html.description.abstract<p>Nuclear Factor One (NFI) transcription factors regulate temporal gene expression required for dendritogenesis and synaptogenesis via delayed occupancy of target promoters in developing cerebellar granule neurons (CGNs). Mechanisms that promote NFI temporal occupancy have not been previously defined. We show here that the transcription factor ETV1 directly binds to and is required for expression and NFI occupancy of a cohort of NFI-dependent genes in CGNs maturing in vivo. Expression of ETV1 is low in early postnatal cerebellum and increases with maturation, mirroring NFI temporal occupancy of coregulated target genes. Precocious expression of ETV1 in mouse CGNs accelerated onset of expression and NFI temporal occupancy of late target genes and enhanced Map2(+) neurite outgrowth. ETV1 also activated expression and NFI occupancy of the Etv1 gene itself, and this autoregulatory loop preceded ETV1 binding and activation of other coregulated target genes in vivo. These findings suggest a potential model in which ETV1 activates NFI temporal binding to a subset of late-expressed genes in a stepwise manner by initial positive feedback regulation of the Etv1 gene itself followed by activation of downstream coregulated targets as ETV1 expression increases. Sequential transcription factor autoregulation and subsequent binding to downstream promoters may provide an intrinsic developmental timer for dendrite/synapse gene expression.</p>
dc.identifier.submissionpathoapubs/2846
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.source.pages1488-99


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Copyright © 2016 Ding et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
Except where otherwise noted, this item's license is described as Copyright © 2016 Ding et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).