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dc.contributor.authorBecker, Pamela S.
dc.contributor.authorLi, Zhifang
dc.contributor.authorPotselueva, Tamara
dc.contributor.authorMadri, Joseph A.
dc.contributor.authorNewburger, Peter E.
dc.contributor.authorBerliner, Nancy
dc.date2022-08-11T08:09:45.000
dc.date.accessioned2022-08-23T16:42:24Z
dc.date.available2022-08-23T16:42:24Z
dc.date.issued1996-07-01
dc.date.submitted2008-04-14
dc.identifier.citationBlood. 1996 Jul 1;88(1):261-7.
dc.identifier.issn0006-4971 (Print)
dc.identifier.pmid8704182
dc.identifier.urihttp://hdl.handle.net/20.500.14038/40086
dc.description.abstractThe promyelocytic leukemia cell line, NB4, carries the t(15; 17) translocation and undergoes limited maturation in response to differentiation agents. Growth on laminin enhanced the ability of all-trans retinoic acid (ATRA) to promote morphologic maturation of these cells. Although exposure to ATRA in suspension yielded minimal maturation beyond the myelocyte stage, after 72 hours of exposure to ATRA on laminin the cells acquired the histologic appearance of metamyelocytes, band forms, and segmented neutrophils. After 96 hours, some cells acquired a spindle shape and became tightly adherent. Growth on collagen types I, III, IV, or fibronectin did not have this effect, although some cells did adhere to fibronectin. NB4 cells treated with ATRA in suspension or on laminin acquired the equivalent ability to reduce nitroblue tetrazolium or cytochrome C. Despite the improved morphologic maturation on laminin, the cells did not express secondary granule proteins such as lactoferrin or neutrophil collagenase. In addition, growth on laminin abolished cell proliferation in the presence of ionomycin. Growth on laminin and/or with ATRA induced new expression of alpha 6 integrin, a laminin receptor, as assessed by reverse transcription-polymerase chain reaction. Different conditions of growth (laminin or differentiation agent) resulted in specific patterns of expression of the alpha 6A and alpha 6B isoforms. Treatment with ATRA also resulted in the acquisition of high-level surface expression of alpha 6 integrin, as assessed by flow cytometry. Thus, treatment of NB4 promyelocytic leukemia cells with ATRA induced expression of alpha 6 integrin (a laminin receptor alpha-chain) and enabled more advanced maturation when the cells were grown on the extracellular matrix component, laminin, compared with tissue culture plastic.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8704182&dopt=Abstract">Link to article in PubMed</a>
dc.subjectAntigens, CD
dc.subjectAntigens, CD29
dc.subjectBase Sequence
dc.subjectCalcium
dc.subjectCell Culture Techniques
dc.subjectCell Differentiation
dc.subjectCell Division
dc.subjectCollagen
dc.subjectDrug Synergism
dc.subjectFibronectins
dc.subjectGene Expression Regulation, Leukemic
dc.subjectHumans
dc.subjectIntegrin alpha6
dc.subjectIonomycin
dc.subjectIonophores
dc.subjectLaminin
dc.subjectLeukemia, Promyelocytic, Acute
dc.subjectMolecular Sequence Data
dc.subjectNeoplasm Proteins
dc.subjectNeoplastic Stem Cells
dc.subjectOxidation-Reduction
dc.subjectPolymerase Chain Reaction
dc.subjectReceptors, Laminin
dc.subjectTretinoin
dc.subjectTumor Cells, Cultured
dc.subjectCancer Biology
dc.subjectCell and Developmental Biology
dc.subjectOncology
dc.subjectPediatrics
dc.titleLaminin promotes differentiation of NB4 promyelocytic leukemia cells with all-trans retinoic acid
dc.typeJournal Article
dc.source.journaltitleBlood
dc.source.volume88
dc.source.issue1
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1288&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/289
dc.identifier.contextkey489626
refterms.dateFOA2022-08-23T16:42:24Z
html.description.abstract<p>The promyelocytic leukemia cell line, NB4, carries the t(15; 17) translocation and undergoes limited maturation in response to differentiation agents. Growth on laminin enhanced the ability of all-trans retinoic acid (ATRA) to promote morphologic maturation of these cells. Although exposure to ATRA in suspension yielded minimal maturation beyond the myelocyte stage, after 72 hours of exposure to ATRA on laminin the cells acquired the histologic appearance of metamyelocytes, band forms, and segmented neutrophils. After 96 hours, some cells acquired a spindle shape and became tightly adherent. Growth on collagen types I, III, IV, or fibronectin did not have this effect, although some cells did adhere to fibronectin. NB4 cells treated with ATRA in suspension or on laminin acquired the equivalent ability to reduce nitroblue tetrazolium or cytochrome C. Despite the improved morphologic maturation on laminin, the cells did not express secondary granule proteins such as lactoferrin or neutrophil collagenase. In addition, growth on laminin abolished cell proliferation in the presence of ionomycin. Growth on laminin and/or with ATRA induced new expression of alpha 6 integrin, a laminin receptor, as assessed by reverse transcription-polymerase chain reaction. Different conditions of growth (laminin or differentiation agent) resulted in specific patterns of expression of the alpha 6A and alpha 6B isoforms. Treatment with ATRA also resulted in the acquisition of high-level surface expression of alpha 6 integrin, as assessed by flow cytometry. Thus, treatment of NB4 promyelocytic leukemia cells with ATRA induced expression of alpha 6 integrin (a laminin receptor alpha-chain) and enabled more advanced maturation when the cells were grown on the extracellular matrix component, laminin, compared with tissue culture plastic.</p>
dc.identifier.submissionpathoapubs/289
dc.contributor.departmentDepartment of Pediatrics
dc.contributor.departmentDepartment of Medicine
dc.source.pages261-7


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