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    Stem cell factor enhances interleukin-3 dependent induction of 68-kD calmodulin-binding protein and thymidine kinase activity in NFS-60 cells

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    Authors
    Reddy, G. Prem Veer
    Quesenberry, Peter J.
    UMass Chan Affiliations
    Cancer Center
    Department of Cell Biology
    Document Type
    Journal Article
    Publication Date
    1996-04-15
    Keywords
    Animals
    Calmodulin-Binding Proteins
    Cell Division
    Culture Media, Serum-Free
    DNA Polymerase II
    Drug Synergism
    Gene Expression Regulation, Leukemic
    Granulocyte-Macrophage Colony-Stimulating Factor
    Hematopoietic Stem Cells
    Humans
    Interleukin-3
    Interleukin-6
    Leukemia, Myeloid
    Mice
    Neoplasm Proteins
    Neoplastic Stem Cells
    Stem Cell Factor
    Thymidine Kinase
    Tumor Cells, Cultured
    Tumor Stem Cell Assay
    Cancer Biology
    Cell and Developmental Biology
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    Abstract
    Stem cell factor (SCF) is known to act synergistically with other hematopoietic factors in increasing the colony formation of hematopoietic progenitor cells. We have shown that interleukin-3 (IL-3)-dependent proliferation of NFS-60 cells is associated with the induction of a specific calmodulin-binding protein of about 68 kD (CaM-BP68). To evaluate the relationship between proliferative stimulation and the induction of CaM-BP68 by cytokines, we examined whether the increased proliferative potential of NFS-60 cells in response to SCF is reflected in an increased induction of the CaM-BP68. We observed that SCF alone has a limited effect on proliferative stimulation and on the induction of CaM-BP68 in factor-deprived NFS-60 cells. However, when combined with IL-3, granulocyte colony-stimulating factor (G-CSF), or IL-6, it caused a significant increase in cytokine-dependent proliferative stimulation, as well as in the induction of CaM-BP68. Furthermore, an increase in IL-3-dependent induction of CaM-BP68 in the presence of SCF coincided with a corresponding increase in thymidine kinase activity, whose expression is linked to G1/S transition of the cells. At low concentrations SCF caused a synergistic increase in IL-3-dependent induction of both CaM-BP68 and thymidine kinase activity. In contrast to the changes in CaM-BP68 and thymidine kinase activity, no significant changes in DNA polymerase alpha were observed in factor-deprived NFS-60 cells in response to IL-3 and/or SCF. These observations suggest an increased expression of CaM-BP68 and thymidine kinase are associated with the synergistic effect of SCF on factor-dependent proliferation of hematopoietic progenitor cells.
    Source
    Blood. 1996 Apr 15;87(8):3195-202.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/40109
    PubMed ID
    8605334
    Related Resources
    Link to article in PubMed
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    UMass Chan Faculty and Researcher Publications

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