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dc.contributor.authorReddy, G. Prem Veer
dc.contributor.authorQuesenberry, Peter J.
dc.date2022-08-11T08:09:46.000
dc.date.accessioned2022-08-23T16:42:31Z
dc.date.available2022-08-23T16:42:31Z
dc.date.issued1996-04-15
dc.date.submitted2008-04-14
dc.identifier.citationBlood. 1996 Apr 15;87(8):3195-202.
dc.identifier.issn0006-4971 (Print)
dc.identifier.pmid8605334
dc.identifier.urihttp://hdl.handle.net/20.500.14038/40109
dc.description.abstractStem cell factor (SCF) is known to act synergistically with other hematopoietic factors in increasing the colony formation of hematopoietic progenitor cells. We have shown that interleukin-3 (IL-3)-dependent proliferation of NFS-60 cells is associated with the induction of a specific calmodulin-binding protein of about 68 kD (CaM-BP68). To evaluate the relationship between proliferative stimulation and the induction of CaM-BP68 by cytokines, we examined whether the increased proliferative potential of NFS-60 cells in response to SCF is reflected in an increased induction of the CaM-BP68. We observed that SCF alone has a limited effect on proliferative stimulation and on the induction of CaM-BP68 in factor-deprived NFS-60 cells. However, when combined with IL-3, granulocyte colony-stimulating factor (G-CSF), or IL-6, it caused a significant increase in cytokine-dependent proliferative stimulation, as well as in the induction of CaM-BP68. Furthermore, an increase in IL-3-dependent induction of CaM-BP68 in the presence of SCF coincided with a corresponding increase in thymidine kinase activity, whose expression is linked to G1/S transition of the cells. At low concentrations SCF caused a synergistic increase in IL-3-dependent induction of both CaM-BP68 and thymidine kinase activity. In contrast to the changes in CaM-BP68 and thymidine kinase activity, no significant changes in DNA polymerase alpha were observed in factor-deprived NFS-60 cells in response to IL-3 and/or SCF. These observations suggest an increased expression of CaM-BP68 and thymidine kinase are associated with the synergistic effect of SCF on factor-dependent proliferation of hematopoietic progenitor cells.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8605334&dopt=Abstract">Link to article in PubMed</a>
dc.subjectAnimals
dc.subjectCalmodulin-Binding Proteins
dc.subjectCell Division
dc.subjectCulture Media, Serum-Free
dc.subjectDNA Polymerase II
dc.subjectDrug Synergism
dc.subjectGene Expression Regulation, Leukemic
dc.subjectGranulocyte-Macrophage Colony-Stimulating Factor
dc.subjectHematopoietic Stem Cells
dc.subjectHumans
dc.subjectInterleukin-3
dc.subjectInterleukin-6
dc.subjectLeukemia, Myeloid
dc.subjectMice
dc.subjectNeoplasm Proteins
dc.subjectNeoplastic Stem Cells
dc.subjectStem Cell Factor
dc.subjectThymidine Kinase
dc.subjectTumor Cells, Cultured
dc.subjectTumor Stem Cell Assay
dc.subjectCancer Biology
dc.subjectCell and Developmental Biology
dc.titleStem cell factor enhances interleukin-3 dependent induction of 68-kD calmodulin-binding protein and thymidine kinase activity in NFS-60 cells
dc.typeJournal Article
dc.source.journaltitleBlood
dc.source.volume87
dc.source.issue8
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1290&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/291
dc.identifier.contextkey489629
refterms.dateFOA2022-08-23T16:42:31Z
html.description.abstract<p>Stem cell factor (SCF) is known to act synergistically with other hematopoietic factors in increasing the colony formation of hematopoietic progenitor cells. We have shown that interleukin-3 (IL-3)-dependent proliferation of NFS-60 cells is associated with the induction of a specific calmodulin-binding protein of about 68 kD (CaM-BP68). To evaluate the relationship between proliferative stimulation and the induction of CaM-BP68 by cytokines, we examined whether the increased proliferative potential of NFS-60 cells in response to SCF is reflected in an increased induction of the CaM-BP68. We observed that SCF alone has a limited effect on proliferative stimulation and on the induction of CaM-BP68 in factor-deprived NFS-60 cells. However, when combined with IL-3, granulocyte colony-stimulating factor (G-CSF), or IL-6, it caused a significant increase in cytokine-dependent proliferative stimulation, as well as in the induction of CaM-BP68. Furthermore, an increase in IL-3-dependent induction of CaM-BP68 in the presence of SCF coincided with a corresponding increase in thymidine kinase activity, whose expression is linked to G1/S transition of the cells. At low concentrations SCF caused a synergistic increase in IL-3-dependent induction of both CaM-BP68 and thymidine kinase activity. In contrast to the changes in CaM-BP68 and thymidine kinase activity, no significant changes in DNA polymerase alpha were observed in factor-deprived NFS-60 cells in response to IL-3 and/or SCF. These observations suggest an increased expression of CaM-BP68 and thymidine kinase are associated with the synergistic effect of SCF on factor-dependent proliferation of hematopoietic progenitor cells.</p>
dc.identifier.submissionpathoapubs/291
dc.contributor.departmentCancer Center
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages3195-202


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