Regulation of X-linked gene expression during early mouse development by Rlim
Shalek, Alex K.
Lawrence, Jeanne B.
Torres, Eduardo M.
Zhu, Lihua J.
Rando, Oliver J.
UMass Chan AffiliationsUMass Metabolic Network
Department of Cell and Developmental Biology
Department of Biochemistry and Molecular Pharmacology
Department of Molecular, Cell, and Cancer Biology
X chromosome inactivation
mouse preimplantation development
single embryo RNA-seq
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AbstractMammalian X-linked gene expression is highly regulated as female cells contain two and male one X chromosome (X). To adjust the X gene dosage between genders, female mouse preimplantation embryos undergo an imprinted form of X chromosome inactivation (iXCI) that requires both Rlim (also known as Rnf12) and the long non-coding RNA Xist. Moreover, it is thought that gene expression from the single active X is upregulated to correct for bi-allelic autosomal (A) gene expression. We have combined mouse genetics with RNA-seq on single mouse embryos to investigate functions of Rlim on the temporal regulation of iXCI and Xist. Our results reveal crucial roles of Rlim for the maintenance of high Xist RNA levels, Xist clouds and X-silencing in female embryos at blastocyst stages, while initial Xist expression appears Rlim-independent. We find further that X/A upregulation is initiated in early male and female preimplantation embryos.
SourceElife. 2016 Sep 19;5. pii: e19127. doi: 10.7554/eLife.19127. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/40146
Related ResourcesLink to Article in PubMed
Rights© 2016, Wang et al.