Show simple item record

dc.contributor.authorAnklesaria, Pervin
dc.contributor.authorGreenberger, Joel S.
dc.contributor.authorFitzgerald, Thomas J.
dc.contributor.authorSullenbarger, Brent A.
dc.contributor.authorWicha, Max S.
dc.contributor.authorCampbell, Alan D.
dc.date2022-08-11T08:09:46.000
dc.date.accessioned2022-08-23T16:43:03Z
dc.date.available2022-08-23T16:43:03Z
dc.date.issued1991-04-15
dc.date.submitted2008-04-14
dc.identifier.citationBlood. 1991 Apr 15;77(8):1691-8.
dc.identifier.issn0006-4971 (Print)
dc.identifier.pmid2015397
dc.identifier.urihttp://hdl.handle.net/20.500.14038/40215
dc.description.abstractMutant Sl/Sld mice exhibit decreased marrow hematopoiesis. The defect is known to reside in the marrow microenvironment of these animals, which is reproduced in vitro by primary marrow explants as well as by cloned marrow stromal cell lines. Bone marrow progenitor cells are incapable of adhering to primary Sl/Sld stromal cells or cloned stromal cell lines derived from them to form cobblestone-islands and proliferate. The role of hemonectin, a marrow-specific adhesion protein in the defective hematopoiesis of the Sl/Sld mice, was studied. Indirect immunoperoxidase staining of marrow in situ from Sl/Sld mice showed little specific staining while specific staining was seen in a pericellular distribution in marrow from +/+ mice. Hemonectin expression in several cloned stromal cell lines from Sl/Sld mice was compared by immunoblotting with that in cloned stromal cell lines from normal +/+ littermates. Cell line Sld3, which has the least hematopoiesis supportive capacity in vitro, showed no detectable hemonectin by immunoblotting, while Sld1 and Sld2 showed detectable but greatly reduced amounts compared with normal +/+ 2.4, GBI/6, and D2XRII. Confluent cultures incubated with purified hemonectin and engrafted with enriched progenitors showed a significant increase in the cumulative number of cobbleston-islands and day 14 spleen colony-forming units (CFU-s) forming progenitors (39.15 +/- 3.6/dish; 16.3 +/- 3.1/dish, respectively), compared with untreated Sld3 cultures (cobblestone-islands 8.1 +/- 3.6/dish; CFU-s forming progenitors 8.8 +/- 0.05/dish). Hemonectin-mediated progenitor cell binding to the Sld3 stromal cells was specifically inhibited by antihemonectin but not by preimmune serum. These data support the role of hemonectin in early progenitor-stromal cell interactions.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=2015397&dopt=Abstract ">Link to article in PubMed</a>
dc.subjectAnimals
dc.subjectCell Adhesion
dc.subjectCell Line
dc.subjectColony-Forming Units Assay
dc.subjectHematopoietic Stem Cells
dc.subjectKinetics
dc.subjectMembrane Proteins
dc.subjectMice
dc.subjectMice, Mutant Strains
dc.subjectCancer Biology
dc.subjectCell and Developmental Biology
dc.titleHemonectin mediates adhesion of engrafted murine progenitors to a clonal bone marrow stromal cell line from Sl/Sld mice
dc.typeJournal Article
dc.source.journaltitleBlood
dc.source.volume77
dc.source.issue8
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1300&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/301
dc.identifier.contextkey489639
refterms.dateFOA2022-08-23T16:43:04Z
html.description.abstract<p>Mutant Sl/Sld mice exhibit decreased marrow hematopoiesis. The defect is known to reside in the marrow microenvironment of these animals, which is reproduced in vitro by primary marrow explants as well as by cloned marrow stromal cell lines. Bone marrow progenitor cells are incapable of adhering to primary Sl/Sld stromal cells or cloned stromal cell lines derived from them to form cobblestone-islands and proliferate. The role of hemonectin, a marrow-specific adhesion protein in the defective hematopoiesis of the Sl/Sld mice, was studied. Indirect immunoperoxidase staining of marrow in situ from Sl/Sld mice showed little specific staining while specific staining was seen in a pericellular distribution in marrow from +/+ mice. Hemonectin expression in several cloned stromal cell lines from Sl/Sld mice was compared by immunoblotting with that in cloned stromal cell lines from normal +/+ littermates. Cell line Sld3, which has the least hematopoiesis supportive capacity in vitro, showed no detectable hemonectin by immunoblotting, while Sld1 and Sld2 showed detectable but greatly reduced amounts compared with normal +/+ 2.4, GBI/6, and D2XRII. Confluent cultures incubated with purified hemonectin and engrafted with enriched progenitors showed a significant increase in the cumulative number of cobbleston-islands and day 14 spleen colony-forming units (CFU-s) forming progenitors (39.15 +/- 3.6/dish; 16.3 +/- 3.1/dish, respectively), compared with untreated Sld3 cultures (cobblestone-islands 8.1 +/- 3.6/dish; CFU-s forming progenitors 8.8 +/- 0.05/dish). Hemonectin-mediated progenitor cell binding to the Sld3 stromal cells was specifically inhibited by antihemonectin but not by preimmune serum. These data support the role of hemonectin in early progenitor-stromal cell interactions.</p>
dc.identifier.submissionpathoapubs/301
dc.contributor.departmentDepartment of Radiation Oncology
dc.source.pages1691-8


Files in this item

Thumbnail
Name:
2015397.pdf
Size:
7.491Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record