Adipose-derived human stem/stromal cells: comparative organ specific mitochondrial bioenergy profiles
Authors
Ferng, Alice S.Marsh, Katherine M.
Fleming, Jamie M.
Conway, Renee F.
Schipper, David
Bajaj, Naing
Connell, Alana M.
Pilikian, Tia
Johnson, Kitsie
Runyan, Ray
Black, Stephen M.
Szivek, John A.
Khalpey, Zain
UMass Chan Affiliations
Department of AnesthesiologyDocument Type
Journal ArticlePublication Date
2016-12-01Keywords
Adipose-derived stem/stromal cellsBioenergetic profiling
Extracellular flux
Human adipose tissue
Mesenchymal stem cells
Mitochondrial bioenergetics
Stromal vascular fraction
Tissue engineering
Cell Biology
Cellular and Molecular Physiology
Metadata
Show full item recordAbstract
BACKGROUND: Adipose-derived stem/stromal cells (ASCs) isolated from the stromal vascular fraction are a source of mesenchymal stem cells that have been shown to be beneficial in many regenerative medicine applications. ASCs are an attractive source of stem cells in particular, due to their lack of immunogenicity. This study examines differences between mitochondrial bioenergetic profiles of ASCs isolated from adipose tissue of five peri-organ regions: pericardial, thymic, knee, shoulder, and abdomen. RESULTS: Flow cytometry showed that the majority of each ASC population isolated from the adipose tissue of 12 donors, with an n = 3 for each tissue type, were positive for MSC markers CD90, CD73, and CD105, and negative for hematopoietic markers CD34, CD11B, CD19, and CD45. Bioenergetic profiles were obtained for ASCs with an n = 4 for each tissue type and graphed together for comparison. Mitochondrial stress tests provided the following measurements: basal respiration rate (measured as oxygen consumption rate [pmol O2/min], ATP production, proton leak, maximal respiration, respiratory control ratio, coupling efficiency, and non-mitochondrial respiration. Glycolytic stress tests provided the following measurements: basal glycolysis rate (measured as extracellular acidification rate [mpH/min]), glycolytic capacity, glycolytic reserve, and non-glycolytic acidification. CONCLUSIONS: The main goal of this manuscript was to provide baseline reference values for future experiments and to compare bioenergetic potentials of ASCs isolated from adipose tissue harvested from different anatomical locations. Through an investigation of mitochondrial respiration and glycolysis, it was demonstrated that bioenergetic profiles do not significantly differ by region due to depot-dependent and donor-dependent variability. Thus, although the physiological function, microenvironment and anatomical harvest site may directly affect the characteristics of ASCs isolated from different organ regions, the ultimate utility of ASCs remains independent of the anatomical harvest site.Source
SpringerPlus. 2016 Dec 1;5(1):2057. eCollection 2016. Link to article on publisher's siteDOI
10.1186/s40064-016-3712-1Permanent Link to this Item
http://hdl.handle.net/20.500.14038/40238PubMed ID
27995034Related Resources
Link to Article in PubMedRights
© The Author(s) 2016.Distribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.1186/s40064-016-3712-1