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dc.contributor.authorFerng, Alice S.
dc.contributor.authorMarsh, Katherine M.
dc.contributor.authorFleming, Jamie M.
dc.contributor.authorConway, Renee F.
dc.contributor.authorSchipper, David
dc.contributor.authorBajaj, Naing
dc.contributor.authorConnell, Alana M.
dc.contributor.authorPilikian, Tia
dc.contributor.authorJohnson, Kitsie
dc.contributor.authorRunyan, Ray
dc.contributor.authorBlack, Stephen M.
dc.contributor.authorSzivek, John A.
dc.contributor.authorKhalpey, Zain
dc.date2022-08-11T08:09:46.000
dc.date.accessioned2022-08-23T16:43:10Z
dc.date.available2022-08-23T16:43:10Z
dc.date.issued2016-12-01
dc.date.submitted2017-05-17
dc.identifier.citationSpringerPlus. 2016 Dec 1;5(1):2057. eCollection 2016. <a href="https://doi.org/10.1186/s40064-016-3712-1">Link to article on publisher's site</a>
dc.identifier.doi10.1186/s40064-016-3712-1
dc.identifier.pmid27995034
dc.identifier.urihttp://hdl.handle.net/20.500.14038/40238
dc.description.abstractBACKGROUND: Adipose-derived stem/stromal cells (ASCs) isolated from the stromal vascular fraction are a source of mesenchymal stem cells that have been shown to be beneficial in many regenerative medicine applications. ASCs are an attractive source of stem cells in particular, due to their lack of immunogenicity. This study examines differences between mitochondrial bioenergetic profiles of ASCs isolated from adipose tissue of five peri-organ regions: pericardial, thymic, knee, shoulder, and abdomen. RESULTS: Flow cytometry showed that the majority of each ASC population isolated from the adipose tissue of 12 donors, with an n = 3 for each tissue type, were positive for MSC markers CD90, CD73, and CD105, and negative for hematopoietic markers CD34, CD11B, CD19, and CD45. Bioenergetic profiles were obtained for ASCs with an n = 4 for each tissue type and graphed together for comparison. Mitochondrial stress tests provided the following measurements: basal respiration rate (measured as oxygen consumption rate [pmol O2/min], ATP production, proton leak, maximal respiration, respiratory control ratio, coupling efficiency, and non-mitochondrial respiration. Glycolytic stress tests provided the following measurements: basal glycolysis rate (measured as extracellular acidification rate [mpH/min]), glycolytic capacity, glycolytic reserve, and non-glycolytic acidification. CONCLUSIONS: The main goal of this manuscript was to provide baseline reference values for future experiments and to compare bioenergetic potentials of ASCs isolated from adipose tissue harvested from different anatomical locations. Through an investigation of mitochondrial respiration and glycolysis, it was demonstrated that bioenergetic profiles do not significantly differ by region due to depot-dependent and donor-dependent variability. Thus, although the physiological function, microenvironment and anatomical harvest site may directly affect the characteristics of ASCs isolated from different organ regions, the ultimate utility of ASCs remains independent of the anatomical harvest site.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=27995034&dopt=Abstract">Link to Article in PubMed</a>
dc.rights© The Author(s) 2016.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAdipose-derived stem/stromal cells
dc.subjectBioenergetic profiling
dc.subjectExtracellular flux
dc.subjectHuman adipose tissue
dc.subjectMesenchymal stem cells
dc.subjectMitochondrial bioenergetics
dc.subjectStromal vascular fraction
dc.subjectTissue engineering
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.titleAdipose-derived human stem/stromal cells: comparative organ specific mitochondrial bioenergy profiles
dc.typeArticle
dc.source.journaltitleSpringerPlus
dc.source.volume5
dc.source.issue1
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=4036&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/3031
dc.identifier.contextkey10176531
refterms.dateFOA2022-08-23T16:43:10Z
html.description.abstract<p>BACKGROUND: Adipose-derived stem/stromal cells (ASCs) isolated from the stromal vascular fraction are a source of mesenchymal stem cells that have been shown to be beneficial in many regenerative medicine applications. ASCs are an attractive source of stem cells in particular, due to their lack of immunogenicity. This study examines differences between mitochondrial bioenergetic profiles of ASCs isolated from adipose tissue of five peri-organ regions: pericardial, thymic, knee, shoulder, and abdomen.</p> <p>RESULTS: Flow cytometry showed that the majority of each ASC population isolated from the adipose tissue of 12 donors, with an n = 3 for each tissue type, were positive for MSC markers CD90, CD73, and CD105, and negative for hematopoietic markers CD34, CD11B, CD19, and CD45. Bioenergetic profiles were obtained for ASCs with an n = 4 for each tissue type and graphed together for comparison. Mitochondrial stress tests provided the following measurements: basal respiration rate (measured as oxygen consumption rate [pmol O2/min], ATP production, proton leak, maximal respiration, respiratory control ratio, coupling efficiency, and non-mitochondrial respiration. Glycolytic stress tests provided the following measurements: basal glycolysis rate (measured as extracellular acidification rate [mpH/min]), glycolytic capacity, glycolytic reserve, and non-glycolytic acidification.</p> <p>CONCLUSIONS: The main goal of this manuscript was to provide baseline reference values for future experiments and to compare bioenergetic potentials of ASCs isolated from adipose tissue harvested from different anatomical locations. Through an investigation of mitochondrial respiration and glycolysis, it was demonstrated that bioenergetic profiles do not significantly differ by region due to depot-dependent and donor-dependent variability. Thus, although the physiological function, microenvironment and anatomical harvest site may directly affect the characteristics of ASCs isolated from different organ regions, the ultimate utility of ASCs remains independent of the anatomical harvest site.</p>
dc.identifier.submissionpathoapubs/3031
dc.contributor.departmentDepartment of Anesthesiology
dc.source.pages2057


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