Characterization of a new oda3 allele, oda3-6, defective in assembly of the outer dynein arm-docking complex in Chlamydomonas reinhardtii
Authors
Brown, Jason M.Mosley, Matthew
Montes-Berrueta, Daniela
Hou, Yuqing
Yang, Fan
Scarbrough, Chasity
Witman, George B.
Wirschell, Maureen
UMass Chan Affiliations
Witman LabDepartment of Radiology
Department of Cell and Developmental Biology
Document Type
Journal ArticlePublication Date
2017-03-14
Metadata
Show full item recordAbstract
We have used an insertional mutagenesis approach to generate new C. reinhardtii motility mutants. Of 56 mutants isolated, one is a new allele at the ODA3 locus, called oda3-6. Similar to the previously characterized oda3 alleles, oda3-6 has a slow-jerky swimming phenotype and reduced swimming speed. The oda3-6 mutant fails to assemble the outer dynein arm motor and outer dynein arm-docking complex (ODA-DC) in the ciliary axoneme due to an insertion in the 5' end of the DCC1 gene, which encodes the DC1 subunit of the ODA-DC. Transformation of oda3-6 with the wild-type DCC1 gene rescues the mutant swimming phenotype and restores assembly of the ODA-DC and the outer dynein arm in the cilium. This is the first oda3 mutant to be characterized at the molecular level and is likely to be very useful for further analysis of DC1 function.Source
PLoS One. 2017 Mar 14;12(3):e0173842. eCollection 2017. Link to article on publisher's siteDOI
10.1371/journal.pone.0173842Permanent Link to this Item
http://hdl.handle.net/20.500.14038/40256PubMed ID
28291812Related Resources
Link to Article in PubMedRights
Copyright: © 2017 Brown et al.Distribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0173842