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dc.contributor.authorMera-Ramirez, Angelica
dc.contributor.authorCastillo, Andres
dc.contributor.authorOrobio, Yenifer
dc.contributor.authorGomez, Maria Adelaida.
dc.contributor.authorGallego-Marin, Carolina
dc.date2022-08-11T08:09:47.000
dc.date.accessioned2022-08-23T16:43:24Z
dc.date.available2022-08-23T16:43:24Z
dc.date.issued2017-02-28
dc.date.submitted2017-06-16
dc.identifier.citationBMC Infect Dis. 2017 Feb 28;17(1):177. doi: 10.1186/s12879-017-2281-4. <a href="https://doi.org/10.1186/s12879-017-2281-4">Link to article on publisher's site</a>
dc.identifier.issn1471-2334 (Linking)
dc.identifier.doi10.1186/s12879-017-2281-4
dc.identifier.pmid28241747
dc.identifier.urihttp://hdl.handle.net/20.500.14038/40286
dc.description.abstractBACKGROUND: Clinical manifestations of cutaneous leishmaniasis (CL) caused by Leishmania (Viannia) range from asymptomatic infection to self-limited, or chronic (non-healing) cutaneous lesions. Given the critical role of the immune response in the clinical outcome of CL, it is plausible that functional polymorphisms in immune-related genes contribute to define the clinical manifestations of human infection. METHODS: DNA samples from a retrospective cohort of individuals from an endemic area of L. V. panamensis transmission in Colombia were used to determine the frequency of SNPs in TNFalpha, IL-10 and TLR4 genes. DNA samples were obtained from 74 adult participants: 38 patients presenting chronic cutaneous leishmaniasis (CCL) and 36 individuals with asymptomatic infection. Genotyping of TNFalpha-308G/A, IL-10-819C/T, and TLR4 Asp299Gly and Thr399Ile SNPs, was conducted by PCR-restriction fragment length polymorphisms. Allele, genotype frequencies and associations between SNPs and clinical groups were evaluated. RESULTS: The A allele in TNFalpha-308G/A SNP was found more frequently in individuals with asymptomatic infection (16% vs 7%), whereas the CC genotype in IL-10-819 C/T SNP was more frequent in patients with CCL (34% vs. 27% in asymptomatic individuals). No differences in allele frequencies for TLR4 SNPs were found among groups. CONCLUSION: This study provides a reference base for statistical power calculation and design of association studies of genetic polymorphisms in immune response related-genes and the pathogenesis of infections caused by L. V. panamensis.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28241747&dopt=Abstract">Link to Article in PubMed</a>
dc.rightsCopyright © The Author(s). 2017.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectsymptomatic infection
dc.subjectchronicity
dc.subjectcutaneous leishmaniasis
dc.subjectsingle nucleotide polymorphism
dc.subjectGenetics and Genomics
dc.subjectImmunology and Infectious Disease
dc.subjectParasitic Diseases
dc.titleScreening of TNFalpha, IL-10 and TLR4 single nucleotide polymorphisms in individuals with asymptomatic and chronic cutaneous leishmaniasis in Colombia: a pilot study
dc.typeJournal Article
dc.source.journaltitleBMC infectious diseases
dc.source.volume17
dc.source.issue1
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=4092&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/3087
dc.identifier.contextkey10314718
refterms.dateFOA2022-08-23T16:43:24Z
html.description.abstract<p>BACKGROUND: Clinical manifestations of cutaneous leishmaniasis (CL) caused by Leishmania (Viannia) range from asymptomatic infection to self-limited, or chronic (non-healing) cutaneous lesions. Given the critical role of the immune response in the clinical outcome of CL, it is plausible that functional polymorphisms in immune-related genes contribute to define the clinical manifestations of human infection.</p> <p>METHODS: DNA samples from a retrospective cohort of individuals from an endemic area of L. V. panamensis transmission in Colombia were used to determine the frequency of SNPs in TNFalpha, IL-10 and TLR4 genes. DNA samples were obtained from 74 adult participants: 38 patients presenting chronic cutaneous leishmaniasis (CCL) and 36 individuals with asymptomatic infection. Genotyping of TNFalpha-308G/A, IL-10-819C/T, and TLR4 Asp299Gly and Thr399Ile SNPs, was conducted by PCR-restriction fragment length polymorphisms. Allele, genotype frequencies and associations between SNPs and clinical groups were evaluated.</p> <p>RESULTS: The A allele in TNFalpha-308G/A SNP was found more frequently in individuals with asymptomatic infection (16% vs 7%), whereas the CC genotype in IL-10-819 C/T SNP was more frequent in patients with CCL (34% vs. 27% in asymptomatic individuals). No differences in allele frequencies for TLR4 SNPs were found among groups.</p> <p>CONCLUSION: This study provides a reference base for statistical power calculation and design of association studies of genetic polymorphisms in immune response related-genes and the pathogenesis of infections caused by L. V. panamensis.</p>
dc.identifier.submissionpathoapubs/3087
dc.contributor.departmentDepartment of Medicine, Division of Infectious Diseases and Immunology
dc.source.pages177


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