Krill Oil-In-Water Emulsion Protects against Lipopolysaccharide-Induced Proinflammatory Activation of Macrophages In Vitro
dc.contributor.author | Bonaterra, Gabriel A. | |
dc.contributor.author | Driscoll, David F. | |
dc.contributor.author | Schwarzbach, Hans | |
dc.contributor.author | Kinscherf, Ralf | |
dc.date | 2022-08-11T08:09:47.000 | |
dc.date.accessioned | 2022-08-23T16:43:32Z | |
dc.date.available | 2022-08-23T16:43:32Z | |
dc.date.issued | 2017-03-15 | |
dc.date.submitted | 2017-09-12 | |
dc.identifier.citation | Mar Drugs. 2017 Mar 15;15(3). pii: E74. doi: 10.3390/md15030074. <a href="https://doi.org/10.3390/md15030074">Link to article on publisher's site</a> | |
dc.identifier.issn | 1660-3397 (Linking) | |
dc.identifier.doi | 10.3390/md15030074 | |
dc.identifier.pmid | 28294970 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/40315 | |
dc.description.abstract | BACKGROUND: Parenteral nutrition is often a mandatory therapeutic strategy for cases of septicemia. Likewise, therapeutic application of anti-oxidants, anti-inflammatory therapy, and endotoxin lowering, by removal or inactivation, might be beneficial to ameliorate the systemic inflammatory response during the acute phases of critical illness. Concerning anti-inflammatory properties in this setting, omega-3 fatty acids of marine origin have been frequently described. This study investigated the anti-inflammatory and LPS-inactivating properties of krill oil (KO)-in-water emulsion in human macrophages in vitro. MATERIALS AND METHODS: Differentiated THP-1 macrophages were activated using specific ultrapure-LPS that binds only on the toll-like receptor 4 (TLR4) in order to determine the inhibitory properties of the KO emulsion on the LPS-binding capacity, and the subsequent release of TNF-alpha. RESULTS: KO emulsion inhibited the macrophage binding of LPS to the TLR4 by 50% (at 12.5 microg/mL) and 75% (at 25 microg/mL), whereas, at 50 microg/mL, completely abolished the LPS binding. Moreover, KO (12.5 microg/mL, 25 microg/mL, or 50 microg/mL) also inhibited (30%, 40%, or 75%, respectively) the TNF-alpha release after activation with 0.01 microg/mL LPS in comparison with LPS treatment alone. CONCLUSION: KO emulsion influences the LPS-induced pro-inflammatory activation of macrophages, possibly due to inactivation of the LPS binding capacity. | |
dc.language.iso | en_US | |
dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28294970&dopt=Abstract">Link to Article in PubMed</a></p> | |
dc.rights | Copyright © 2017 by the authors. | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | LPS | |
dc.subject | cytokines | |
dc.subject | krill oil-in-water emulsion | |
dc.subject | omega-3 fatty acids | |
dc.subject | phospholipids | |
dc.subject | septic shock | |
dc.subject | Chemicals and Drugs | |
dc.subject | Medicinal Chemistry and Pharmaceutics | |
dc.subject | Pharmacy and Pharmaceutical Sciences | |
dc.title | Krill Oil-In-Water Emulsion Protects against Lipopolysaccharide-Induced Proinflammatory Activation of Macrophages In Vitro | |
dc.type | Journal Article | |
dc.source.journaltitle | Marine drugs | |
dc.source.volume | 15 | |
dc.source.issue | 3 | |
dc.identifier.legacyfulltext | https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=4122&context=oapubs&unstamped=1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/oapubs/3116 | |
dc.identifier.contextkey | 10737547 | |
refterms.dateFOA | 2022-08-23T16:43:32Z | |
html.description.abstract | <p>BACKGROUND: Parenteral nutrition is often a mandatory therapeutic strategy for cases of septicemia. Likewise, therapeutic application of anti-oxidants, anti-inflammatory therapy, and endotoxin lowering, by removal or inactivation, might be beneficial to ameliorate the systemic inflammatory response during the acute phases of critical illness. Concerning anti-inflammatory properties in this setting, omega-3 fatty acids of marine origin have been frequently described. This study investigated the anti-inflammatory and LPS-inactivating properties of krill oil (KO)-in-water emulsion in human macrophages in vitro.</p> <p>MATERIALS AND METHODS: Differentiated THP-1 macrophages were activated using specific ultrapure-LPS that binds only on the toll-like receptor 4 (TLR4) in order to determine the inhibitory properties of the KO emulsion on the LPS-binding capacity, and the subsequent release of TNF-alpha.</p> <p>RESULTS: KO emulsion inhibited the macrophage binding of LPS to the TLR4 by 50% (at 12.5 microg/mL) and 75% (at 25 microg/mL), whereas, at 50 microg/mL, completely abolished the LPS binding. Moreover, KO (12.5 microg/mL, 25 microg/mL, or 50 microg/mL) also inhibited (30%, 40%, or 75%, respectively) the TNF-alpha release after activation with 0.01 microg/mL LPS in comparison with LPS treatment alone.</p> <p>CONCLUSION: KO emulsion influences the LPS-induced pro-inflammatory activation of macrophages, possibly due to inactivation of the LPS binding capacity.</p> | |
dc.identifier.submissionpath | oapubs/3116 | |
dc.contributor.department | Department of Medicine, Division of Gastroenterology | |
dc.source.pages | 74 |