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dc.contributor.authorAshique, A. M.
dc.contributor.authorHart, L. S.
dc.contributor.authorThomas, C. D. L.
dc.contributor.authorClement, J. G.
dc.contributor.authorPivonka, P.
dc.contributor.authorCarter, Yasmin
dc.contributor.authorMousseau, D. D.
dc.contributor.authorCooper, D. M. L.
dc.date2022-08-11T08:09:48.000
dc.date.accessioned2022-08-23T16:43:47Z
dc.date.available2022-08-23T16:43:47Z
dc.date.issued2017-06-28
dc.date.submitted2017-11-01
dc.identifier.citationBone Rep. 2017 Jun 28;7:9-16. doi: 10.1016/j.bonr.2017.06.002. eCollection 2017 Dec. <a href="https://doi.org/10.1016/j.bonr.2017.06.002">Link to article on publisher's site</a>
dc.identifier.issn2352-1872 (Linking)
dc.identifier.doi10.1016/j.bonr.2017.06.002
dc.identifier.pmid28752112
dc.identifier.urihttp://hdl.handle.net/20.500.14038/40370
dc.description.abstractThe lacunar-canalicular network (LCN) of bone contains osteocytes and their dendritic extensions, which allow for intercellular communication, and are believed to serve as the mechanosensors that coordinate the processes of bone modeling and remodeling. Imbalances in remodeling, for example, are linked to bone disease, including fragility associated with aging. We have reported that there is a reduction in scale for one component of the LCN, osteocyte lacunar volume, across the human lifespan in females. In the present study, we explore the hypothesis that canalicular porosity also declines with age. To visualize the LCN and to determine how its components are altered with aging, we examined samples from young (age: 20-23 y; n = 5) and aged (age: 70-86 y; n = 6) healthy women donors utilizing a fluorescent labelling technique in combination with confocal laser scanning microscopy. A large cross-sectional area of cortical bone spanning the endosteal to periosteal surfaces from the anterior proximal femoral shaft was examined in order to account for potential trans-cortical variation in the LCN. Overall, we found that LCN areal fraction was reduced by 40.6% in the samples from aged women. This reduction was due, in part, to a reduction in lacunar density (21.4% decline in lacunae number per given area of bone), but much more so due to a 44.6% decline in canalicular areal fraction. While the areal fraction of larger vascular canals was higher in endosteal vs. periosteal regions for both age groups, no regional differences were observed in the areal fractions of the LCN and its components for either age group. Our data indicate that the LCN is diminished in aged women, and is largely due to a decline in the canalicular areal fraction, and that, unlike vascular canal porosity, this diminished LCN is uniform across the cortex.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28752112&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1016/j.bonr.2017.06.002
dc.rightsCopyright 2017 The Authors.
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBone
dc.subjectLacunae
dc.subjectCanaliculi
dc.subjectHuman aging
dc.subjectConfocal laser scanning microscopy
dc.subjectMusculoskeletal Diseases
dc.subjectMusculoskeletal System
dc.subjectRadiology
dc.titleLacunar-canalicular network in femoral cortical bone is reduced in aged women and is predominantly due to a loss of canalicular porosity
dc.typeJournal Article
dc.source.journaltitleBone reports
dc.source.volume7
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=4177&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/3169
dc.identifier.contextkey10981252
refterms.dateFOA2022-08-23T16:43:48Z
html.description.abstract<p>The lacunar-canalicular network (LCN) of bone contains osteocytes and their dendritic extensions, which allow for intercellular communication, and are believed to serve as the mechanosensors that coordinate the processes of bone modeling and remodeling. Imbalances in remodeling, for example, are linked to bone disease, including fragility associated with aging. We have reported that there is a reduction in scale for one component of the LCN, osteocyte lacunar volume, across the human lifespan in females. In the present study, we explore the hypothesis that canalicular porosity also declines with age. To visualize the LCN and to determine how its components are altered with aging, we examined samples from young (age: 20-23 y; n = 5) and aged (age: 70-86 y; n = 6) healthy women donors utilizing a fluorescent labelling technique in combination with confocal laser scanning microscopy. A large cross-sectional area of cortical bone spanning the endosteal to periosteal surfaces from the anterior proximal femoral shaft was examined in order to account for potential trans-cortical variation in the LCN. Overall, we found that LCN areal fraction was reduced by 40.6% in the samples from aged women. This reduction was due, in part, to a reduction in lacunar density (21.4% decline in lacunae number per given area of bone), but much more so due to a 44.6% decline in canalicular areal fraction. While the areal fraction of larger vascular canals was higher in endosteal vs. periosteal regions for both age groups, no regional differences were observed in the areal fractions of the LCN and its components for either age group. Our data indicate that the LCN is diminished in aged women, and is largely due to a decline in the canalicular areal fraction, and that, unlike vascular canal porosity, this diminished LCN is uniform across the cortex.</p>
dc.identifier.submissionpathoapubs/3169
dc.contributor.departmentDepartment of Radiology
dc.source.pages9-16


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