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dc.contributor.authorWillcox, Abby
dc.contributor.authorRichardson, Sarah J.
dc.contributor.authorWalker, Lucy S. K.
dc.contributor.authorKent, Sally C
dc.contributor.authorMorgan, Noel G.
dc.contributor.authorGillespie, Kathleen M.
dc.date2022-08-11T08:09:48.000
dc.date.accessioned2022-08-23T16:43:59Z
dc.date.available2022-08-23T16:43:59Z
dc.date.issued2017-07-01
dc.date.submitted2017-12-22
dc.identifier.citationDiabetologia. 2017 Jul;60(7):1294-1303. doi: 10.1007/s00125-017-4221-7. Epub 2017 Feb 17. <a href="https://doi.org/10.1007/s00125-017-4221-7">Link to article on publisher's site</a>
dc.identifier.issn0012-186X (Linking)
dc.identifier.doi10.1007/s00125-017-4221-7
dc.identifier.pmid28213757
dc.identifier.urihttp://hdl.handle.net/20.500.14038/40411
dc.description.abstractAIMS/HYPOTHESIS: Pancreatic lymph nodes (PLNs) are critical sites for the initial interaction between islet autoantigens and autoreactive lymphocytes, but the histology of PLNs in tissue from individuals with type 1 diabetes has not been analysed in detail. The aim of this study was to examine PLN tissue sections from healthy donors compared with those at risk of, or with recent-onset and longer-duration type 1 diabetes. METHODS: Immunofluorescence staining was used to examine PLN sections from the following donor groups: non-diabetic (n=15), non-diabetic islet autoantibody-positive (n=5), recent-onset ( < /=1.5 years duration) type 1 diabetes (n=13), and longer-duration type 1 diabetes (n=15). Staining for CD3, CD20 and Ki67 was used to detect primary and secondary (germinal centre-containing) follicles and CD21 and CD35 to detect follicular dendritic cell networks. RESULTS: The frequency of secondary follicles was lower in the recent-onset type 1 diabetes group compared with the non-diabetic control group. The presence of insulitis (as evidence of ongoing beta cell destruction) and diagnosis of type 1 diabetes at a younger age, however, did not appear to be associated with a lower frequency of secondary follicles. A higher proportion of primary B cell follicles were observed to lack follicular dendritic cell networks in the recent-onset type 1 diabetes group. CONCLUSIONS/INTERPRETATION: Histological analysis of rare PLNs from individuals with type 1 diabetes suggests a previously unrecognised phenotype comprising decreased primary B cell follicle frequency and fewer follicular dendritic cell networks in recent-onset type 1 diabetes.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28213757&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487689/
dc.rights© The Author(s) 2017. This article is published with open access at Springerlink.com
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHistology
dc.subjectIslet autoimmunity
dc.subjectPancreatic lymph nodes
dc.subjectType 1 diabetes
dc.subjectEndocrine System Diseases
dc.subjectEndocrinology, Diabetes, and Metabolism
dc.subjectImmune System Diseases
dc.subjectNutritional and Metabolic Diseases
dc.titleGerminal centre frequency is decreased in pancreatic lymph nodes from individuals with recent-onset type 1 diabetes
dc.typeJournal Article
dc.source.journaltitleDiabetologia
dc.source.volume60
dc.source.issue7
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=4226&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/3218
dc.identifier.contextkey11293149
refterms.dateFOA2022-08-23T16:43:59Z
html.description.abstract<p>AIMS/HYPOTHESIS: Pancreatic lymph nodes (PLNs) are critical sites for the initial interaction between islet autoantigens and autoreactive lymphocytes, but the histology of PLNs in tissue from individuals with type 1 diabetes has not been analysed in detail. The aim of this study was to examine PLN tissue sections from healthy donors compared with those at risk of, or with recent-onset and longer-duration type 1 diabetes.</p> <p>METHODS: Immunofluorescence staining was used to examine PLN sections from the following donor groups: non-diabetic (n=15), non-diabetic islet autoantibody-positive (n=5), recent-onset ( < /=1.5 years duration) type 1 diabetes (n=13), and longer-duration type 1 diabetes (n=15). Staining for CD3, CD20 and Ki67 was used to detect primary and secondary (germinal centre-containing) follicles and CD21 and CD35 to detect follicular dendritic cell networks.</p> <p>RESULTS: The frequency of secondary follicles was lower in the recent-onset type 1 diabetes group compared with the non-diabetic control group. The presence of insulitis (as evidence of ongoing beta cell destruction) and diagnosis of type 1 diabetes at a younger age, however, did not appear to be associated with a lower frequency of secondary follicles. A higher proportion of primary B cell follicles were observed to lack follicular dendritic cell networks in the recent-onset type 1 diabetes group.</p> <p>CONCLUSIONS/INTERPRETATION: Histological analysis of rare PLNs from individuals with type 1 diabetes suggests a previously unrecognised phenotype comprising decreased primary B cell follicle frequency and fewer follicular dendritic cell networks in recent-onset type 1 diabetes.</p>
dc.identifier.submissionpathoapubs/3218
dc.contributor.departmentDivision of Diabetes, Department of Medicine, Diabetes Center of Excellence,
dc.source.pages1294-1303


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© The Author(s) 2017. This article is published with open access at Springerlink.com
Except where otherwise noted, this item's license is described as © The Author(s) 2017. This article is published with open access at Springerlink.com