Germinal centre frequency is decreased in pancreatic lymph nodes from individuals with recent-onset type 1 diabetes
dc.contributor.author | Willcox, Abby | |
dc.contributor.author | Richardson, Sarah J. | |
dc.contributor.author | Walker, Lucy S. K. | |
dc.contributor.author | Kent, Sally C | |
dc.contributor.author | Morgan, Noel G. | |
dc.contributor.author | Gillespie, Kathleen M. | |
dc.date | 2022-08-11T08:09:48.000 | |
dc.date.accessioned | 2022-08-23T16:43:59Z | |
dc.date.available | 2022-08-23T16:43:59Z | |
dc.date.issued | 2017-07-01 | |
dc.date.submitted | 2017-12-22 | |
dc.identifier.citation | Diabetologia. 2017 Jul;60(7):1294-1303. doi: 10.1007/s00125-017-4221-7. Epub 2017 Feb 17. <a href="https://doi.org/10.1007/s00125-017-4221-7">Link to article on publisher's site</a> | |
dc.identifier.issn | 0012-186X (Linking) | |
dc.identifier.doi | 10.1007/s00125-017-4221-7 | |
dc.identifier.pmid | 28213757 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/40411 | |
dc.description.abstract | AIMS/HYPOTHESIS: Pancreatic lymph nodes (PLNs) are critical sites for the initial interaction between islet autoantigens and autoreactive lymphocytes, but the histology of PLNs in tissue from individuals with type 1 diabetes has not been analysed in detail. The aim of this study was to examine PLN tissue sections from healthy donors compared with those at risk of, or with recent-onset and longer-duration type 1 diabetes. METHODS: Immunofluorescence staining was used to examine PLN sections from the following donor groups: non-diabetic (n=15), non-diabetic islet autoantibody-positive (n=5), recent-onset ( < /=1.5 years duration) type 1 diabetes (n=13), and longer-duration type 1 diabetes (n=15). Staining for CD3, CD20 and Ki67 was used to detect primary and secondary (germinal centre-containing) follicles and CD21 and CD35 to detect follicular dendritic cell networks. RESULTS: The frequency of secondary follicles was lower in the recent-onset type 1 diabetes group compared with the non-diabetic control group. The presence of insulitis (as evidence of ongoing beta cell destruction) and diagnosis of type 1 diabetes at a younger age, however, did not appear to be associated with a lower frequency of secondary follicles. A higher proportion of primary B cell follicles were observed to lack follicular dendritic cell networks in the recent-onset type 1 diabetes group. CONCLUSIONS/INTERPRETATION: Histological analysis of rare PLNs from individuals with type 1 diabetes suggests a previously unrecognised phenotype comprising decreased primary B cell follicle frequency and fewer follicular dendritic cell networks in recent-onset type 1 diabetes. | |
dc.language.iso | en_US | |
dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28213757&dopt=Abstract">Link to Article in PubMed</a></p> | |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487689/ | |
dc.rights | © The Author(s) 2017. This article is published with open access at Springerlink.com | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Histology | |
dc.subject | Islet autoimmunity | |
dc.subject | Pancreatic lymph nodes | |
dc.subject | Type 1 diabetes | |
dc.subject | Endocrine System Diseases | |
dc.subject | Endocrinology, Diabetes, and Metabolism | |
dc.subject | Immune System Diseases | |
dc.subject | Nutritional and Metabolic Diseases | |
dc.title | Germinal centre frequency is decreased in pancreatic lymph nodes from individuals with recent-onset type 1 diabetes | |
dc.type | Journal Article | |
dc.source.journaltitle | Diabetologia | |
dc.source.volume | 60 | |
dc.source.issue | 7 | |
dc.identifier.legacyfulltext | https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=4226&context=oapubs&unstamped=1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/oapubs/3218 | |
dc.identifier.contextkey | 11293149 | |
refterms.dateFOA | 2022-08-23T16:43:59Z | |
html.description.abstract | <p>AIMS/HYPOTHESIS: Pancreatic lymph nodes (PLNs) are critical sites for the initial interaction between islet autoantigens and autoreactive lymphocytes, but the histology of PLNs in tissue from individuals with type 1 diabetes has not been analysed in detail. The aim of this study was to examine PLN tissue sections from healthy donors compared with those at risk of, or with recent-onset and longer-duration type 1 diabetes.</p> <p>METHODS: Immunofluorescence staining was used to examine PLN sections from the following donor groups: non-diabetic (n=15), non-diabetic islet autoantibody-positive (n=5), recent-onset ( < /=1.5 years duration) type 1 diabetes (n=13), and longer-duration type 1 diabetes (n=15). Staining for CD3, CD20 and Ki67 was used to detect primary and secondary (germinal centre-containing) follicles and CD21 and CD35 to detect follicular dendritic cell networks.</p> <p>RESULTS: The frequency of secondary follicles was lower in the recent-onset type 1 diabetes group compared with the non-diabetic control group. The presence of insulitis (as evidence of ongoing beta cell destruction) and diagnosis of type 1 diabetes at a younger age, however, did not appear to be associated with a lower frequency of secondary follicles. A higher proportion of primary B cell follicles were observed to lack follicular dendritic cell networks in the recent-onset type 1 diabetes group.</p> <p>CONCLUSIONS/INTERPRETATION: Histological analysis of rare PLNs from individuals with type 1 diabetes suggests a previously unrecognised phenotype comprising decreased primary B cell follicle frequency and fewer follicular dendritic cell networks in recent-onset type 1 diabetes.</p> | |
dc.identifier.submissionpath | oapubs/3218 | |
dc.contributor.department | Division of Diabetes, Department of Medicine, Diabetes Center of Excellence, | |
dc.source.pages | 1294-1303 |