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dc.contributor.authorDanielson, Kirsty M.
dc.contributor.authorTanriverdi, Kahraman
dc.contributor.authorFreedman, Jane E.
dc.contributor.authorDas, Saumya
dc.date2022-08-11T08:09:50.000
dc.date.accessioned2022-08-23T16:45:28Z
dc.date.available2022-08-23T16:45:28Z
dc.date.issued2018-06-01
dc.date.submitted2018-07-27
dc.identifier.citation<p>EBioMedicine. 2018 Jun;32:172-181. doi: 10.1016/j.ebiom.2018.05.013. Epub 2018 May 18. <a href="https://doi.org/10.1016/j.ebiom.2018.05.013">Link to article on publisher's site</a></p>
dc.identifier.issn2352-3964 (Linking)
dc.identifier.doi10.1016/j.ebiom.2018.05.013
dc.identifier.pmid29779700
dc.identifier.urihttp://hdl.handle.net/20.500.14038/40689
dc.description<p>Full list of authors omitted for brevity. For full list see article.</p>
dc.description.abstractDespite substantial declines in mortality following myocardial infarction (MI), subsequent left ventricular remodeling (LVRm) remains a significant long-term complication. Extracellular small non-coding RNAs (exRNAs) have been associated with cardiac inflammation and fibrosis and we hypothesized that they are associated with post-MI LVRm phenotypes. RNA sequencing of exRNAs was performed on plasma samples from patients with "beneficial" (decrease LVESVI > /=20%, n=11) and "adverse" (increase LVESVI > /=15%, n=11) LVRm. Selected differentially expressed exRNAs were validated by RT-qPCR (n=331) and analyzed for their association with LVRm determined by cardiac MRI. Principal components of exRNAs were associated with LVRm phenotypes post-MI; specifically, LV mass, LV ejection fraction, LV end systolic volume index, and fibrosis. We then investigated the temporal regulation and cellular origin of exRNAs in murine and cell models and found that: 1) plasma and tissue miRNA expression was temporally regulated; 2) the majority of the miRNAs were increased acutely in tissue and at sub-acute or chronic time-points in plasma; 3) miRNA expression was cell-specific; and 4) cardiomyocytes release a subset of the identified miRNAs packaged in exosomes into culture media in response to hypoxia/reoxygenation. In conclusion, we find that plasma exRNAs are temporally regulated and are associated with measures of post-MI LVRm.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=29779700&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright © 2018 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectinflammation
dc.subjectcardiac magnetic resonance imaging
dc.subjectextracellular RNA
dc.subjectleft ventricular remodeling
dc.subjectmyocardial infarction
dc.subjectRNA sequencing
dc.subjectmicroRNA
dc.subjectCardiology
dc.subjectCardiovascular Diseases
dc.subjectCell Biology
dc.titlePlasma Circulating Extracellular RNAs in Left Ventricular Remodeling Post-Myocardial Infarction
dc.typeJournal Article
dc.source.journaltitleEBioMedicine
dc.source.volume32
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=4503&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/3492
dc.identifier.contextkey12556179
refterms.dateFOA2022-08-23T16:45:28Z
html.description.abstract<p>Despite substantial declines in mortality following myocardial infarction (MI), subsequent left ventricular remodeling (LVRm) remains a significant long-term complication. Extracellular small non-coding RNAs (exRNAs) have been associated with cardiac inflammation and fibrosis and we hypothesized that they are associated with post-MI LVRm phenotypes. RNA sequencing of exRNAs was performed on plasma samples from patients with "beneficial" (decrease LVESVI > /=20%, n=11) and "adverse" (increase LVESVI > /=15%, n=11) LVRm. Selected differentially expressed exRNAs were validated by RT-qPCR (n=331) and analyzed for their association with LVRm determined by cardiac MRI. Principal components of exRNAs were associated with LVRm phenotypes post-MI; specifically, LV mass, LV ejection fraction, LV end systolic volume index, and fibrosis. We then investigated the temporal regulation and cellular origin of exRNAs in murine and cell models and found that: 1) plasma and tissue miRNA expression was temporally regulated; 2) the majority of the miRNAs were increased acutely in tissue and at sub-acute or chronic time-points in plasma; 3) miRNA expression was cell-specific; and 4) cardiomyocytes release a subset of the identified miRNAs packaged in exosomes into culture media in response to hypoxia/reoxygenation. In conclusion, we find that plasma exRNAs are temporally regulated and are associated with measures of post-MI LVRm.</p>
dc.identifier.submissionpathoapubs/3492
dc.contributor.departmentDepartment of Medicine, Division of Cardiovascular Medicine
dc.source.pages172-181


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Copyright © 2018 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Except where otherwise noted, this item's license is described as Copyright © 2018 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).