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dc.contributor.authorTian, Yonghao
dc.contributor.authorChen, Hanxiang
dc.contributor.authorQiao, Lijun
dc.contributor.authorZhang, Wenhao
dc.contributor.authorZheng, Jingyi
dc.contributor.authorZhao, Weiming
dc.contributor.authorChen, Jason J.
dc.contributor.authorZhang, Weifang
dc.date2022-08-11T08:09:50.000
dc.date.accessioned2022-08-23T16:45:35Z
dc.date.available2022-08-23T16:45:35Z
dc.date.issued2018-06-12
dc.date.submitted2018-08-08
dc.identifier.citation<p>J Cell Mol Med. 2018 Jun 12. doi: 10.1111/jcmm.13693. <a href="https://doi.org/10.1111/jcmm.13693">Link to article on publisher's site</a></p>
dc.identifier.issn1582-1838 (Linking)
dc.identifier.doi10.1111/jcmm.13693
dc.identifier.pmid29893470
dc.identifier.urihttp://hdl.handle.net/20.500.14038/40712
dc.description.abstractInfection with high-risk human papillomaviruses (HR-HPVs, including HPV-16, HPV-18, HPV-31) plays a central aetiologic role in the development of cervical carcinoma. The transforming properties of HR-HPVs mainly reside in viral oncoproteins E6 and E7. E6 protein degrades the tumour suppressor p53 and abrogates cell cycle checkpoints. Cancerous inhibitor of protein phosphatase 2A (CIP2A) is an oncoprotein that is involved in the carcinogenesis of many human malignancies. Our previous data showed that CIP2A was overexpressed in cervical cancer. However, the regulation of CIP2A by HPV-16E6 remains to be elucidated. In this study, we demonstrated that HPV-16E6 significantly up-regulated CIP2A mRNA and protein expression in a p53-degradation-dependent manner. Knockdown of CIP2A by siRNA inhibited viability and DNA synthesis and caused G1 cell cycle arrest of 16E6-expressing cells. Knockdown of CIP2A resulted in a significant reduction in the expression of cyclin-dependent kinase 1 (Cdk1) and Cdk2. Although CIP2A has been reported to stabilize c-Myc by inhibiting PP2A-mediated dephosphorylation of c-Myc, we have presented evidence that the regulation of Cdk1 and Cdk2 by CIP2A is dependent on transcription factor B-Myb rather than c-Myc. Taken together, our study reveals the role of CIP2A in abrogating the G1 checkpoint in HPV-16E6-expressing cells and helps in understanding the molecular basis of HPV-induced oncogenesis.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=29893470&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rights© 2018 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectB-Myb
dc.subjectCIP2A
dc.subjectCdk1
dc.subjectE6 oncoprotein
dc.subjectG1/S transition
dc.subjecthuman papillomavirus
dc.subjectCancer Biology
dc.subjectCell Biology
dc.subjectImmunology and Infectious Disease
dc.subjectMolecular Biology
dc.subjectNeoplasms
dc.titleCIP2A facilitates the G1/S cell cycle transition via B-Myb in human papillomavirus 16 oncoprotein E6-expressing cells
dc.typeJournal Article
dc.source.journaltitleJournal of cellular and molecular medicine
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=4526&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/3515
dc.identifier.contextkey12624499
refterms.dateFOA2022-08-23T16:45:36Z
html.description.abstract<p>Infection with high-risk human papillomaviruses (HR-HPVs, including HPV-16, HPV-18, HPV-31) plays a central aetiologic role in the development of cervical carcinoma. The transforming properties of HR-HPVs mainly reside in viral oncoproteins E6 and E7. E6 protein degrades the tumour suppressor p53 and abrogates cell cycle checkpoints. Cancerous inhibitor of protein phosphatase 2A (CIP2A) is an oncoprotein that is involved in the carcinogenesis of many human malignancies. Our previous data showed that CIP2A was overexpressed in cervical cancer. However, the regulation of CIP2A by HPV-16E6 remains to be elucidated. In this study, we demonstrated that HPV-16E6 significantly up-regulated CIP2A mRNA and protein expression in a p53-degradation-dependent manner. Knockdown of CIP2A by siRNA inhibited viability and DNA synthesis and caused G1 cell cycle arrest of 16E6-expressing cells. Knockdown of CIP2A resulted in a significant reduction in the expression of cyclin-dependent kinase 1 (Cdk1) and Cdk2. Although CIP2A has been reported to stabilize c-Myc by inhibiting PP2A-mediated dephosphorylation of c-Myc, we have presented evidence that the regulation of Cdk1 and Cdk2 by CIP2A is dependent on transcription factor B-Myb rather than c-Myc. Taken together, our study reveals the role of CIP2A in abrogating the G1 checkpoint in HPV-16E6-expressing cells and helps in understanding the molecular basis of HPV-induced oncogenesis.</p>
dc.identifier.submissionpathoapubs/3515
dc.contributor.departmentDepartment of Medicine


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© 2018 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as © 2018 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.