LLC tumor cells-derivated factors reduces adipogenesis in co-culture system
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Authors
Lopes, Magno AlvesOliveira Franco, Felipe
Henriques, Felipe
Peres, Sidney Barnabe
Batista, Miguel Luiz Jr.
UMass Chan Affiliations
Program in Molecular MedicineDocument Type
Journal ArticlePublication Date
2018-07-30Keywords
Cancer researchCell biology
Molecular biology
Amino Acids, Peptides, and Proteins
Cancer Biology
Cell Biology
Cells
Cellular and Molecular Physiology
Developmental Biology
Genetic Phenomena
Molecular Biology
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Show full item recordAbstract
Cancer cachexia (CC) is a multifactorial syndrome with an unknown etiology. The primary symptom is the progressive reduction of the body weight. Recently, down-regulation of adipogenic and lipogenic genes were demonstrated to be early affected during cachexia progression in adipose tissue (AT), resulting in AT remodeling. Thus, this study aimed to evaluate in a co-culture system the influence of the Lewis Lung Carcinoma (LLC) tumor cells (c/c-LLC) in an established pre-adipocyte cell line 3T3-L1 adipogenic capacity. c/c-LLC in the presence of 3T3-L1 caused a reduction in lipids accumulation, suggesting that secretory tumor cells products may affect adipogenesis. Interestingly, a very early (day 2) down-regulation of proliferator-activated receptor gamma (PPARgamma) and CCAAT/enhancer-binding protein alpha (C/EBPalpha), followed by late genes (day 4 and 8), adiponectin, perilipin, and fatty acid-binding protein 4 (FABP4). Caspase-3 expression was increased on the last day of cell differentiation; it occurred in the expression of pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-alpha). Overall, our results suggest that LLC secretory products impair adipocyte differentiation in a co-culture system and increased apoptosis. In summary, our study has shown the inhibition of the adipogenic process in the 3T3-L1 co-culture system with LLC cells.Source
Heliyon. 2018 Jul 30;4(7):e00708. doi: 10.1016/j.heliyon.2018.e00708. eCollection 2018 Jul. Link to article on publisher's site
DOI
10.1016/j.heliyon.2018.e00708Permanent Link to this Item
http://hdl.handle.net/20.500.14038/40739PubMed ID
30094378Related Resources
Rights
Copyright 2018 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Distribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.1016/j.heliyon.2018.e00708
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Except where otherwise noted, this item's license is described as Copyright 2018 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license
(http://creativecommons.org/licenses/by/4.0/).
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