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dc.contributor.authorFram, Robert J.
dc.contributor.authorCrockett, Jay
dc.contributor.authorVolkert, Michael R.
dc.date2022-08-11T08:09:52.000
dc.date.accessioned2022-08-23T16:47:00Z
dc.date.available2022-08-23T16:47:00Z
dc.date.issued1988-09-01
dc.date.submitted2008-06-18
dc.identifier.citationCancer Res. 1988 Sep 1;48(17):4823-6.
dc.identifier.issn0008-5472 (Print)
dc.identifier.pmid3044578
dc.identifier.urihttp://hdl.handle.net/20.500.14038/40993
dc.description.abstractPrevious work has demonstrated heterogeneous effects of methylating agents on induction of DNA damage inducible genes in Escherichia coli. These studies employed E. coli mutants that have fusions of the lac operon to genes induced by treatment with sublethal levels of alkylating agents. These mutants were selected from random insertions of the Mu-dl (Apr lac) phage by screening for induction of beta-galactosidase activity in the presence of methylmethanesulfonate or N-methyl-N'-nitro-N-nitrosoguanidine. The current report extends these findings by analyzing gene expression caused by mechlorethamine, chloroethylnitrosoureas and cis-diamminedichloroplatinum(II) (cis-DDP). The results demonstrate heterogeneous effects by these agents on gene expression. While 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea induces alkA, other nitrosoureas, mechlorethamine, and cis-DDP do not cause expression of this gene. Further, while all nitrosoureas caused expression of aidC, mechlorethamine and cis-DDP did not. Lastly, cis-DDP caused marked expression of a sulA fusion mutant while not inducing any of the other E. coli fusion mutants.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=3044578&dopt=Abstract">Link to article in PubMed</a>
dc.subjectAlkylating Agents
dc.subjectCisplatin
dc.subjectDNA Repair
dc.subjectDNA, Bacterial
dc.subjectEscherichia coli
dc.subjectGene Expression Regulation
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleGene expression caused by alkylating agents and cis-diamminedichloroplatinum(II) in Escherichia coli
dc.typeJournal Article
dc.source.journaltitleCancer research
dc.source.volume48
dc.source.issue17
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1378&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/379
dc.identifier.contextkey533091
refterms.dateFOA2022-08-23T16:47:00Z
html.description.abstract<p>Previous work has demonstrated heterogeneous effects of methylating agents on induction of DNA damage inducible genes in Escherichia coli. These studies employed E. coli mutants that have fusions of the lac operon to genes induced by treatment with sublethal levels of alkylating agents. These mutants were selected from random insertions of the Mu-dl (Apr lac) phage by screening for induction of beta-galactosidase activity in the presence of methylmethanesulfonate or N-methyl-N'-nitro-N-nitrosoguanidine. The current report extends these findings by analyzing gene expression caused by mechlorethamine, chloroethylnitrosoureas and cis-diamminedichloroplatinum(II) (cis-DDP). The results demonstrate heterogeneous effects by these agents on gene expression. While 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea induces alkA, other nitrosoureas, mechlorethamine, and cis-DDP do not cause expression of this gene. Further, while all nitrosoureas caused expression of aidC, mechlorethamine and cis-DDP did not. Lastly, cis-DDP caused marked expression of a sulA fusion mutant while not inducing any of the other E. coli fusion mutants.</p>
dc.identifier.submissionpathoapubs/379
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.contributor.departmentDepartment of Medicine
dc.source.pages4823-6


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