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dc.contributor.authorMatijasevic, Zdenka
dc.contributor.authorStering, Allen
dc.contributor.authorNiu, Tian-Qi
dc.contributor.authorAustin-Ritchie, Paula
dc.contributor.authorLudlum, David B.
dc.date2022-08-11T08:09:53.000
dc.date.accessioned2022-08-23T16:47:16Z
dc.date.available2022-08-23T16:47:16Z
dc.date.issued1996-10-01
dc.date.submitted2008-06-18
dc.identifier.citation<p>Carcinogenesis. 1996 Oct;17(10):2249-52.</p>
dc.identifier.issn0143-3334 (Print)
dc.identifier.pmid8895496
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41047
dc.description.abstractThe toxic effects of sulfur mustard have been attributed to DNA modification with the formation of 7-hydroxyethylthioethyl guanine, 3-hydroxyethylthioethyl adenine and the cross-link, di-(2-guanin-7-yl-ethyl)sulfide. To investigate the action of bacterial 3-methyladenine DNA glycosylase II (Gly II) on these adducts, calf thymus DNA was modified with [14C]sulfur mustard and used as a substrate for Gly II. Gly II releases both 3-hydroxyethylthioethyl adenine and 7-hydroxyethylthioethyl guanine from this substrate. In comparison with the activity of Gly II towards methylated DNA, 3-hydroxyethylthioethyl adenine is released somewhat more slowly than 3-methyladenine, while 7-hydroxyethylthioethyl guanine is released much more readily than 7-methylguanine. Glycosylase action may play a role in protecting cells from the toxic effects of sulfur mustard.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8895496&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1093/carcin/17.10.2249
dc.subjectDNA Adducts
dc.subject*DNA Glycosylases
dc.subjectDNA Repair
dc.subjectEscherichia coli
dc.subject*Mustard Gas
dc.subjectN-Glycosyl Hydrolases
dc.subjectSubstrate Specificity
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleRelease of sulfur mustard-modified DNA bases by Escherichia coli 3-methyladenine DNA glycosylase II
dc.typeJournal Article
dc.source.journaltitleCarcinogenesis
dc.source.volume17
dc.source.issue10
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/384
dc.identifier.contextkey533096
html.description.abstract<p>The toxic effects of sulfur mustard have been attributed to DNA modification with the formation of 7-hydroxyethylthioethyl guanine, 3-hydroxyethylthioethyl adenine and the cross-link, di-(2-guanin-7-yl-ethyl)sulfide. To investigate the action of bacterial 3-methyladenine DNA glycosylase II (Gly II) on these adducts, calf thymus DNA was modified with [14C]sulfur mustard and used as a substrate for Gly II. Gly II releases both 3-hydroxyethylthioethyl adenine and 7-hydroxyethylthioethyl guanine from this substrate. In comparison with the activity of Gly II towards methylated DNA, 3-hydroxyethylthioethyl adenine is released somewhat more slowly than 3-methyladenine, while 7-hydroxyethylthioethyl guanine is released much more readily than 7-methylguanine. Glycosylase action may play a role in protecting cells from the toxic effects of sulfur mustard.</p>
dc.identifier.submissionpathoapubs/384
dc.contributor.departmentDepartment of Cell Biology
dc.contributor.departmentDepartment of Pharmacology and Molecular Toxicology
dc.source.pages2249-52


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