Plasma indoleamine 2,3-dioxygenase activity as a potential biomarker for early diagnosis of multidrug-resistant tuberculosis in tuberculosis patients
dc.contributor.author | Shi, Wen | |
dc.contributor.author | Wu, Juan | |
dc.contributor.author | Tan, Qi | |
dc.contributor.author | Hu, Chun-Mei | |
dc.contributor.author | Zhang, Xia | |
dc.contributor.author | Pan, Hong-Qiu | |
dc.contributor.author | Yang, Zhen | |
dc.contributor.author | He, Meng-Yu | |
dc.contributor.author | Yu, Min | |
dc.contributor.author | Zhang, Bo | |
dc.contributor.author | Xie, Wei-Ping | |
dc.contributor.author | Wang, Hong | |
dc.date | 2022-08-11T08:09:53.000 | |
dc.date.accessioned | 2022-08-23T16:47:19Z | |
dc.date.available | 2022-08-23T16:47:19Z | |
dc.date.issued | 2019-05-14 | |
dc.date.submitted | 2019-06-21 | |
dc.identifier.citation | <p>Infect Drug Resist. 2019 May 14;12:1265-1276. doi: 10.2147/IDR.S202369. eCollection 2019. <a href="https://doi.org/10.2147/IDR.S202369">Link to article on publisher's site</a></p> | |
dc.identifier.issn | 1178-6973 (Linking) | |
dc.identifier.doi | 10.2147/IDR.S202369 | |
dc.identifier.pmid | 31190914 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/41055 | |
dc.description.abstract | Purpose: Multidrug-resistant tuberculosis (MDR-TB) remains a challenge of global TB control, with difficulty in early detection of drug-sensitive tuberculosis (DS-TB). We investigate the diagnostic significance of IDO as a potential biomarker to discriminate MDR patients among the TB patients. Patients and methods: Plasma indoleamine 2,3-dioxygenase (IDO) was measured by the ratio of kynurenine (Kyn) to tryptophan (Trp) concentrations, using high performance liquid chromatography-mass spectrometry (LC-MS/MS). Chest computed tomography (CT) imaging signs from TB patients were collected and analyzed in 18 DS-TB patients, 16 MDR-TB patients, 6 lung cancer (LC) patients, and 11 healthy individuals. Lung imaging signs from TB patients were collected and analyzed. Results: We found that plasma IDO activity was significantly higher in the MDR-TB patients than in the DS-TB patients (p=0.012) and in the LC patients (p=0.003). We evaluated the diagnostic significance of plasma IDO activity in discriminating the MDR-TB group from the DS-TB group using a receiver operating characteristic (ROC) curve. With a cutoff level of 46.58 uM/mM, the diagnostic sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) for IDO activity were 87.50%, 72.22%, 73.68%, and 86.67%, respectively. Plasma IDO activity was higher in cavity cases than in non-cavity cases (p=0.042), proving a positive correlation between lung cavity number and cavity size (p < 0.05, separately) among all the TB patients studied. Conclusion: Our findings confirmed that plasma IDO activity might have an auxiliary diagnosis value for early discrimination of MDR-TB patients from DS-TB patients. Among the TB patients with cavitary lung lesions, higher plasma IDO activity can indicate a higher risk of MDR-TB. | |
dc.language.iso | en_US | |
dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=31190914&dopt=Abstract">Link to Article in PubMed</a></p> | |
dc.rights | Copyright © 2019 Shi et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/ | |
dc.subject | IDO | |
dc.subject | LC-MS/MS | |
dc.subject | MDR-TB | |
dc.subject | cavitary lung lesion | |
dc.subject | Bacterial Infections and Mycoses | |
dc.subject | Biological Factors | |
dc.subject | Diagnosis | |
dc.subject | Enzymes and Coenzymes | |
dc.subject | Immunology and Infectious Disease | |
dc.subject | Therapeutics | |
dc.title | Plasma indoleamine 2,3-dioxygenase activity as a potential biomarker for early diagnosis of multidrug-resistant tuberculosis in tuberculosis patients | |
dc.type | Journal Article | |
dc.source.journaltitle | Infection and drug resistance | |
dc.source.volume | 12 | |
dc.identifier.legacyfulltext | https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=4862&context=oapubs&unstamped=1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/oapubs/3847 | |
dc.identifier.contextkey | 14784755 | |
refterms.dateFOA | 2022-08-23T16:47:19Z | |
html.description.abstract | <p>Purpose: Multidrug-resistant tuberculosis (MDR-TB) remains a challenge of global TB control, with difficulty in early detection of drug-sensitive tuberculosis (DS-TB). We investigate the diagnostic significance of IDO as a potential biomarker to discriminate MDR patients among the TB patients.</p> <p>Patients and methods: Plasma indoleamine 2,3-dioxygenase (IDO) was measured by the ratio of kynurenine (Kyn) to tryptophan (Trp) concentrations, using high performance liquid chromatography-mass spectrometry (LC-MS/MS). Chest computed tomography (CT) imaging signs from TB patients were collected and analyzed in 18 DS-TB patients, 16 MDR-TB patients, 6 lung cancer (LC) patients, and 11 healthy individuals. Lung imaging signs from TB patients were collected and analyzed.</p> <p>Results: We found that plasma IDO activity was significantly higher in the MDR-TB patients than in the DS-TB patients (p=0.012) and in the LC patients (p=0.003). We evaluated the diagnostic significance of plasma IDO activity in discriminating the MDR-TB group from the DS-TB group using a receiver operating characteristic (ROC) curve. With a cutoff level of 46.58 uM/mM, the diagnostic sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) for IDO activity were 87.50%, 72.22%, 73.68%, and 86.67%, respectively. Plasma IDO activity was higher in cavity cases than in non-cavity cases (p=0.042), proving a positive correlation between lung cavity number and cavity size (p < 0.05, separately) among all the TB patients studied.</p> <p>Conclusion: Our findings confirmed that plasma IDO activity might have an auxiliary diagnosis value for early discrimination of MDR-TB patients from DS-TB patients. Among the TB patients with cavitary lung lesions, higher plasma IDO activity can indicate a higher risk of MDR-TB.</p> | |
dc.identifier.submissionpath | oapubs/3847 | |
dc.contributor.department | Department of Population and Quantitative Health Sciences | |
dc.source.pages | 1265-1276 |