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Mick, Eric O.
Corkrey, Heather A.
Tanriverdi, Selim E.
Keeler, Allison M.
Ezzaty Mirhashemi, Marzieh
Elmallah, Mai K.
Freedman, Jane E.
UMass Chan AffiliationsHorae Gene Therapy Center
Department of Pediatrics, Division of Pulmonary Medicine
Department of Population and Quantitative Health Sciences
Department of Medicine, Division of Cardiovascular Medicine
Document TypeJournal Article
Genetics and Genomics
Nucleic Acids, Nucleotides, and Nucleosides
Respiratory Tract Diseases
MetadataShow full item record
AbstractThe presence of nonhuman RNAs in man has been questioned and it is unclear if food-derived miRNAs cross into the circulation. In a large population study, we found nonhuman miRNAs in plasma by RNA sequencing and validated a small number of pine-pollen miRNAs by RT-qPCR in 2,776 people. The presence of these pine-pollen miRNAs associated with hay fever and not with overt cardiovascular or pulmonary disease. Using in vivo and in vitro models, we found that transmission of pollen-miRNAs into the circulation occurs via pulmonary transfer and this transfer was mediated by platelet-pulmonary vascular cell interactions and platelet pollen-DNA uptake. These data demonstrate that pollen-derived plant miRNAs can be horizontally transferred into the circulation via the pulmonary system in humans. Although these data suggest mechanistic plausibility for pulmonary-mediated plant-derived miRNA transfer into the human circulation, our large observational cohort data do not implicate major disease or risk factor association.
iScience. 2019 Sep 27;19:916-926. doi: 10.1016/j.isci.2019.08.035. Epub 2019 Aug 24. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/41196
RightsCopyright 2019 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Except where otherwise noted, this item's license is described as Copyright 2019 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).