Show simple item record

dc.contributor.authorKamga, Larisa
dc.contributor.authorGil, Anna
dc.contributor.authorSong, InYoung
dc.contributor.authorBrody, Robin M.
dc.contributor.authorGhersi, Dario
dc.contributor.authorAslan, Nuray
dc.contributor.authorStern, Lawrence J.
dc.contributor.authorSelin, Liisa K.
dc.contributor.authorLuzuriaga, Katherine
dc.date2022-08-11T08:09:54.000
dc.date.accessioned2022-08-23T16:48:20Z
dc.date.available2022-08-23T16:48:20Z
dc.date.issued2019-11-25
dc.date.submitted2019-12-03
dc.identifier.citation<p>PLoS Pathog. 2019 Nov 25;15(11):e1008122. doi: 10.1371/journal.ppat.1008122. [Epub ahead of print] <a href="https://doi.org/10.1371/journal.ppat.1008122">Link to article on publisher's site</a></p>
dc.identifier.issn1553-7366 (Linking)
dc.identifier.doi10.1371/journal.ppat.1008122
dc.identifier.pmid31765434
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41255
dc.description.abstractThe T cell receptor (TCR) repertoire is an essential component of the CD8 T-cell immune response. Here, we seek to investigate factors that drive selection of TCR repertoires specific to the HLA-A2-restricted immunodominant epitope BRLF1109-117 (YVLDHLIVV) over the course of primary Epstein Barr virus (EBV) infection. Using single-cell paired TCRalphabeta sequencing of tetramer sorted CD8 T cells ex vivo, we show at the clonal level that recognition of the HLA-A2-restricted BRLF1 (YVL-BR, BRLF-1109) epitope is mainly driven by the TCRalpha chain. For the first time, we identify a CDR3alpha (complementarity determining region 3 alpha) motif, KDTDKL, resulting from an obligate AV8.1-AJ34 pairing that was shared by all four individuals studied. This observation coupled with the fact that this public AV8.1-KDTDKL-AJ34 TCR pairs with multiple different TCRbeta chains within the same donor (median 4; range: 1-9), suggests that there are some unique structural features of the interaction between the YVL-BR/MHC and the AV8.1-KDTDKL-AJ34 TCR that leads to this high level of selection. Newly developed TCR motif algorithms identified a lysine at position 1 of the CDR3alpha motif that is highly conserved and likely important for antigen recognition. Crystal structure analysis of the YVL-BR/HLA-A2 complex revealed that the MHC-bound peptide bulges at position 4, exposing a negatively charged aspartic acid that may interact with the positively charged lysine of CDR3alpha. TCR cloning and site-directed mutagenesis of the CDR3alpha lysine ablated YVL-BR-tetramer staining and substantially reduced CD69 upregulation on TCR mutant-transduced cells following antigen-specific stimulation. Reduced activation of T cells expressing this CDR3 motif was also observed following exposure to mutated (D4A) peptide. In summary, we show that a highly public TCR repertoire to an immunodominant epitope of a common human virus is almost completely selected on the basis of CDR3alpha and provide a likely structural basis for the selection. These studies emphasize the importance of examining TCRalpha, as well as TCRbeta, in understanding the CD8 T cell receptor repertoire.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=31765434&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright: © 2019 Kamga et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium,provided the original author and source are credited
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCytotoxic T cells
dc.subjectT cells
dc.subjectSequence motif analysis
dc.subjectT cell receptors
dc.subjectCrystal structure
dc.subjectCell staining
dc.subjectCloning
dc.subjectSequence analysis
dc.subjectUMCCTS funding
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectComputational Biology
dc.subjectImmunology of Infectious Disease
dc.subjectImmunopathology
dc.subjectInfectious Disease
dc.subjectVirology
dc.subjectVirus Diseases
dc.subjectViruses
dc.titleCDR3alpha drives selection of the immunodominant Epstein Barr virus (EBV) BRLF1-specific CD8 T cell receptor repertoire in primary infection
dc.typeJournal Article
dc.source.journaltitlePLoS pathogens
dc.source.volume15
dc.source.issue11
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5060&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4041
dc.identifier.contextkey15909649
refterms.dateFOA2022-08-23T16:48:20Z
html.description.abstract<p>The T cell receptor (TCR) repertoire is an essential component of the CD8 T-cell immune response. Here, we seek to investigate factors that drive selection of TCR repertoires specific to the HLA-A2-restricted immunodominant epitope BRLF1109-117 (YVLDHLIVV) over the course of primary Epstein Barr virus (EBV) infection. Using single-cell paired TCRalphabeta sequencing of tetramer sorted CD8 T cells ex vivo, we show at the clonal level that recognition of the HLA-A2-restricted BRLF1 (YVL-BR, BRLF-1109) epitope is mainly driven by the TCRalpha chain. For the first time, we identify a CDR3alpha (complementarity determining region 3 alpha) motif, KDTDKL, resulting from an obligate AV8.1-AJ34 pairing that was shared by all four individuals studied. This observation coupled with the fact that this public AV8.1-KDTDKL-AJ34 TCR pairs with multiple different TCRbeta chains within the same donor (median 4; range: 1-9), suggests that there are some unique structural features of the interaction between the YVL-BR/MHC and the AV8.1-KDTDKL-AJ34 TCR that leads to this high level of selection. Newly developed TCR motif algorithms identified a lysine at position 1 of the CDR3alpha motif that is highly conserved and likely important for antigen recognition. Crystal structure analysis of the YVL-BR/HLA-A2 complex revealed that the MHC-bound peptide bulges at position 4, exposing a negatively charged aspartic acid that may interact with the positively charged lysine of CDR3alpha. TCR cloning and site-directed mutagenesis of the CDR3alpha lysine ablated YVL-BR-tetramer staining and substantially reduced CD69 upregulation on TCR mutant-transduced cells following antigen-specific stimulation. Reduced activation of T cells expressing this CDR3 motif was also observed following exposure to mutated (D4A) peptide. In summary, we show that a highly public TCR repertoire to an immunodominant epitope of a common human virus is almost completely selected on the basis of CDR3alpha and provide a likely structural basis for the selection. These studies emphasize the importance of examining TCRalpha, as well as TCRbeta, in understanding the CD8 T cell receptor repertoire.</p>
dc.identifier.submissionpathoapubs/4041
dc.contributor.departmentDepartment of Pathology
dc.contributor.departmentProgram in Molecular Medicine
dc.source.pagese1008122


Files in this item

Thumbnail
Name:
journal.ppat.1008122.pdf
Size:
15.96Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record

Copyright: © 2019 Kamga et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium,provided the original author and source are credited
Except where otherwise noted, this item's license is described as Copyright: © 2019 Kamga et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium,provided the original author and source are credited