Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno-Associated Virus
Tai, Phillip W. L.
Mendonca, Craig A.
UMass Chan AffiliationsLi Weibo Institute for Rare Diseases Research
Department of Microbiology and Physiological Systems
Horae Gene Therapy Center
Document TypeJournal Article
adeno‐associated virus (AAV)
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Genetics and Genomics
Research Methods in Life Sciences
MetadataShow full item record
AbstractDelivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno-associated viruses (rAAVs). Together with previous reports from the authors of this paper and others, this approach is potentially applicable to a broad range of mammals. In addition to genome editing and animal modeling, this rAAV-based method can facilitate gene function studies in early-stage embryos.
Adv Sci (Weinh). 2019 Sep 4;6(21):1900440. doi: 10.1002/advs.201900440. eCollection 2019 Nov 6. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/41261
Rights© 2019 The Authors. Published by WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as © 2019 The Authors. Published by WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.