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dc.contributor.authorWang, Dan
dc.contributor.authorNiu, Yuyu
dc.contributor.authorRen, Lingzhi
dc.contributor.authorKang, Yu
dc.contributor.authorTai, Phillip W. L.
dc.contributor.authorSi, Chenyang
dc.contributor.authorMendonca, Craig A.
dc.contributor.authorMa, Hong
dc.contributor.authorGao, Guangping
dc.contributor.authorJi, Weizhi
dc.date2022-08-11T08:09:54.000
dc.date.accessioned2022-08-23T16:48:22Z
dc.date.available2022-08-23T16:48:22Z
dc.date.issued2019-09-04
dc.date.submitted2019-12-09
dc.identifier.citation<p>Adv Sci (Weinh). 2019 Sep 4;6(21):1900440. doi: 10.1002/advs.201900440. eCollection 2019 Nov 6. <a href="https://doi.org/10.1002/advs.201900440">Link to article on publisher's site</a></p>
dc.identifier.issn2198-3844 (Linking)
dc.identifier.doi10.1002/advs.201900440
dc.identifier.pmid31728271
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41261
dc.description.abstractDelivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno-associated viruses (rAAVs). Together with previous reports from the authors of this paper and others, this approach is potentially applicable to a broad range of mammals. In addition to genome editing and animal modeling, this rAAV-based method can facilitate gene function studies in early-stage embryos.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=31728271&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rights© 2019 The Authors. Published by WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCRISPR
dc.subjectadeno‐associated virus (AAV)
dc.subjectanimal modeling
dc.subjectgenome editing
dc.subjectAnalytical, Diagnostic and Therapeutic Techniques and Equipment
dc.subjectGenetics and Genomics
dc.subjectResearch Methods in Life Sciences
dc.subjectViruses
dc.titleGene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno-Associated Virus
dc.typeJournal Article
dc.source.journaltitleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)
dc.source.volume6
dc.source.issue21
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5067&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4048
dc.identifier.contextkey15956218
refterms.dateFOA2022-08-23T16:48:22Z
html.description.abstract<p>Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno-associated viruses (rAAVs). Together with previous reports from the authors of this paper and others, this approach is potentially applicable to a broad range of mammals. In addition to genome editing and animal modeling, this rAAV-based method can facilitate gene function studies in early-stage embryos.</p>
dc.identifier.submissionpathoapubs/4048
dc.contributor.departmentLi Weibo Institute for Rare Diseases Research
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.contributor.departmentHorae Gene Therapy Center
dc.source.pages1900440


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© 2019 The Authors. Published by WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as © 2019 The Authors. Published by WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.