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dc.contributor.authorTian, Qiyu
dc.contributor.authorZhao, Junxing
dc.contributor.authorYang, Qiyuan
dc.contributor.authorWang, Bo
dc.contributor.authorDeavila, Jeanene M.
dc.contributor.authorZhu, Mei-Jun
dc.contributor.authorDu, Min
dc.date2022-08-11T08:09:54.000
dc.date.accessioned2022-08-23T16:48:23Z
dc.date.available2022-08-23T16:48:23Z
dc.date.issued2019-11-06
dc.date.submitted2019-12-09
dc.identifier.citation<p>Aging Cell. 2019 Nov 6:e13059. doi: 10.1111/acel.13059. [Epub ahead of print] <a href="https://doi.org/10.1111/acel.13059">Link to article on publisher's site</a></p>
dc.identifier.issn1474-9718 (Linking)
dc.identifier.doi10.1111/acel.13059
dc.identifier.pmid31691468
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41262
dc.description.abstractAging usually involves the progressive development of certain illnesses, including diabetes and obesity. Due to incapacity to form new white adipocytes, adipose expansion in aged mice primarily depends on adipocyte hypertrophy, which induces metabolic dysfunction. On the other hand, brown adipose tissue burns fatty acids, preventing ectopic lipid accumulation and metabolic diseases. However, the capacity of brown/beige adipogenesis declines inevitably during the aging process. Previously, we reported that DNA demethylation in the Prdm16 promoter is required for beige adipogenesis. DNA methylation is mediated by ten-eleven family proteins (TET) using alpha-ketoglutarate (AKG) as a cofactor. Here, we demonstrated that the circulatory AKG concentration was reduced in middle-aged mice (10-month-old) compared with young mice (2-month-old). Through AKG administration replenishing the AKG pool, aged mice were associated with the lower body weight gain and fat mass, and improved glucose tolerance after challenged with high-fat diet (HFD). These metabolic changes are accompanied by increased expression of brown adipose genes and proteins in inguinal adipose tissue. Cold-induced brown/beige adipogenesis was impeded in HFD mice, whereas AKG rescued the impairment of beige adipocyte functionality in middle-aged mice. Besides, AKG administration up-regulated Prdm16 expression, which was correlated with an increase of DNA demethylation in the Prdm16 promoter. In summary, AKG supplementation promotes beige adipogenesis and alleviates HFD-induced obesity in middle-aged mice, which is associated with enhanced DNA demethylation of the Prdm16 gene.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=31691468&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rights© 2019 The Authors. Aging Cell published by the Anatomical Society and John Wiley and Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDNA demethylation
dc.subjectadipose tissue browning
dc.subjectaging
dc.subjectalpha-ketoglutarate
dc.subjectglucose tolerance
dc.subjecthigh-fat diet
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.subjectLipids
dc.subjectNutritional and Metabolic Diseases
dc.subjectPhysiological Processes
dc.titleDietary alpha-ketoglutarate promotes beige adipogenesis and prevents obesity in middle-aged mice
dc.typeJournal Article
dc.source.journaltitleAging cell
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5068&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4049
dc.identifier.contextkey15956219
refterms.dateFOA2022-08-23T16:48:23Z
html.description.abstract<p>Aging usually involves the progressive development of certain illnesses, including diabetes and obesity. Due to incapacity to form new white adipocytes, adipose expansion in aged mice primarily depends on adipocyte hypertrophy, which induces metabolic dysfunction. On the other hand, brown adipose tissue burns fatty acids, preventing ectopic lipid accumulation and metabolic diseases. However, the capacity of brown/beige adipogenesis declines inevitably during the aging process. Previously, we reported that DNA demethylation in the Prdm16 promoter is required for beige adipogenesis. DNA methylation is mediated by ten-eleven family proteins (TET) using alpha-ketoglutarate (AKG) as a cofactor. Here, we demonstrated that the circulatory AKG concentration was reduced in middle-aged mice (10-month-old) compared with young mice (2-month-old). Through AKG administration replenishing the AKG pool, aged mice were associated with the lower body weight gain and fat mass, and improved glucose tolerance after challenged with high-fat diet (HFD). These metabolic changes are accompanied by increased expression of brown adipose genes and proteins in inguinal adipose tissue. Cold-induced brown/beige adipogenesis was impeded in HFD mice, whereas AKG rescued the impairment of beige adipocyte functionality in middle-aged mice. Besides, AKG administration up-regulated Prdm16 expression, which was correlated with an increase of DNA demethylation in the Prdm16 promoter. In summary, AKG supplementation promotes beige adipogenesis and alleviates HFD-induced obesity in middle-aged mice, which is associated with enhanced DNA demethylation of the Prdm16 gene.</p>
dc.identifier.submissionpathoapubs/4049
dc.contributor.departmentDepartment of Molecular, Cell and Cancer Biology
dc.source.pagese13059


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© 2019 The Authors. Aging Cell published by the Anatomical Society and John Wiley and Sons Ltd.  This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as © 2019 The Authors. Aging Cell published by the Anatomical Society and John Wiley and Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.