Drosophila PSI controls circadian period and the phase of circadian behavior under temperature cycle via tim splicing
AuthorsFoley, Lauren E.
Joshi, Radhika Sudhir
Student AuthorsJinli Ling
Document TypeJournal Article
RNA binding proteins
Amino Acids, Peptides, and Proteins
Neuroscience and Neurobiology
Nucleic Acids, Nucleotides, and Nucleosides
MetadataShow full item record
AbstractThe Drosophila circadian pacemaker consists of transcriptional feedback loops subjected to post-transcriptional and post-translational regulation. While post-translational regulatory mechanisms have been studied in detail, much less is known about circadian post-transcriptional control. Thus, we targeted 364 RNA binding and RNA associated proteins with RNA interference. Among the 43 hits we identified was the alternative splicing regulator P-element somatic inhibitor (PSI). PSI regulates the thermosensitive alternative splicing of timeless (tim), promoting splicing events favored at warm temperature over those increased at cold temperature. Psi downregulation shortens the period of circadian rhythms and advances the phase of circadian behavior under temperature cycle. Interestingly, both phenotypes were suppressed in flies that could produce TIM proteins only from a transgene that cannot form the thermosensitive splicing isoforms. Therefore, we conclude that PSI regulates the period of Drosophila circadian rhythms and circadian behavior phase during temperature cycling through its modulation of the tim splicing pattern.
Elife. 2019 Nov 8;8. pii: 50063. doi: 10.7554/eLife.50063. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/41271
RightsCopyright Foley et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Except where otherwise noted, this item's license is described as Copyright Foley et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.