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dc.contributor.authorFoley, Lauren E.
dc.contributor.authorLing, Jinli
dc.contributor.authorJoshi, Radhika Sudhir
dc.contributor.authorEvantal, Naveh
dc.contributor.authorKadener, Sebastian
dc.contributor.authorEmery, Patrick
dc.date2022-08-11T08:09:54.000
dc.date.accessioned2022-08-23T16:48:26Z
dc.date.available2022-08-23T16:48:26Z
dc.date.issued2019-11-08
dc.date.submitted2019-12-11
dc.identifier.citation<p>Elife. 2019 Nov 8;8. pii: 50063. doi: 10.7554/eLife.50063. <a href="https://doi.org/10.7554/eLife.50063">Link to article on publisher's site</a></p>
dc.identifier.issn2050-084X (Linking)
dc.identifier.doi10.7554/eLife.50063
dc.identifier.pmid31702555
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41271
dc.description.abstractThe Drosophila circadian pacemaker consists of transcriptional feedback loops subjected to post-transcriptional and post-translational regulation. While post-translational regulatory mechanisms have been studied in detail, much less is known about circadian post-transcriptional control. Thus, we targeted 364 RNA binding and RNA associated proteins with RNA interference. Among the 43 hits we identified was the alternative splicing regulator P-element somatic inhibitor (PSI). PSI regulates the thermosensitive alternative splicing of timeless (tim), promoting splicing events favored at warm temperature over those increased at cold temperature. Psi downregulation shortens the period of circadian rhythms and advances the phase of circadian behavior under temperature cycle. Interestingly, both phenotypes were suppressed in flies that could produce TIM proteins only from a transgene that cannot form the thermosensitive splicing isoforms. Therefore, we conclude that PSI regulates the period of Drosophila circadian rhythms and circadian behavior phase during temperature cycling through its modulation of the tim splicing pattern.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=31702555&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright Foley et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectD. melanogaster
dc.subjectRNA binding proteins
dc.subjectalternative splicing
dc.subjectcircadian rhythms
dc.subjectneuroscience
dc.subjecttimeless
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectNeuroscience and Neurobiology
dc.subjectNucleic Acids, Nucleotides, and Nucleosides
dc.titleDrosophila PSI controls circadian period and the phase of circadian behavior under temperature cycle via tim splicing
dc.typeJournal Article
dc.source.journaltitleeLife
dc.source.volume8
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5078&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4059
dc.identifier.contextkey15973593
refterms.dateFOA2022-08-23T16:48:26Z
html.description.abstract<p>The Drosophila circadian pacemaker consists of transcriptional feedback loops subjected to post-transcriptional and post-translational regulation. While post-translational regulatory mechanisms have been studied in detail, much less is known about circadian post-transcriptional control. Thus, we targeted 364 RNA binding and RNA associated proteins with RNA interference. Among the 43 hits we identified was the alternative splicing regulator P-element somatic inhibitor (PSI). PSI regulates the thermosensitive alternative splicing of timeless (tim), promoting splicing events favored at warm temperature over those increased at cold temperature. Psi downregulation shortens the period of circadian rhythms and advances the phase of circadian behavior under temperature cycle. Interestingly, both phenotypes were suppressed in flies that could produce TIM proteins only from a transgene that cannot form the thermosensitive splicing isoforms. Therefore, we conclude that PSI regulates the period of Drosophila circadian rhythms and circadian behavior phase during temperature cycling through its modulation of the tim splicing pattern.</p>
dc.identifier.submissionpathoapubs/4059
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.contributor.departmentEmery Lab
dc.contributor.departmentNeurobiology
dc.source.pagese50063
dc.contributor.studentJinli Ling
dc.contributor.studentLauren Foley
dc.description.thesisprogramNeuroscience


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Copyright Foley et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Except where otherwise noted, this item's license is described as Copyright Foley et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.